miR-215 functions as an oncogene in high-grade glioma by regulating retinoblastoma 1

Biotechnol Lett. 2017 Sep;39(9):1351-1358. doi: 10.1007/s10529-017-2373-7. Epub 2017 Jun 1.

Abstract

Objectives: To investigate the roles of miR-215 in high-grade glioma and to clarify the regulation of retinoblastoma 1 (RB1) by miR-215.

Results: miR-215 is frequently up-regulated in high-grade glioma tissues. Increased miR-215 expression is significantly associated with World Health Organization grade (P < 0.01) tumor size (P < 0.05) and poor prognosis (P < 0.01). Over-expression of miR-215 promoted cell proliferation and knockdown of miR-215 inhibited cell proliferation in vitro. RB1 was identified as a direct and functional target of miR-215. RB1 is generally down-regulated in glioma tissues and its expression inversely correlated with miR-215, which is up-regulated in high-grade glioma tissues, and its expression was negatively correlated with miR-215.

Conclusions: The new miR-215/RB1 axis provides new insights into the molecular mechanism and treatment for glioma.

Keywords: Glioma; High-grade glioma; Proliferation; Retinoblastoma; Tumors; miR-215.

MeSH terms

  • Cell Line, Tumor
  • Cell Proliferation
  • Gene Expression
  • Gene Expression Profiling
  • Glioma / pathology*
  • Glioma / physiopathology*
  • Humans
  • MicroRNAs / metabolism*
  • Retinoblastoma Binding Proteins / metabolism*
  • Ubiquitin-Protein Ligases / metabolism*

Substances

  • MIRN215 microRNA, human
  • MicroRNAs
  • RB1 protein, human
  • Retinoblastoma Binding Proteins
  • Ubiquitin-Protein Ligases