Abstract
Cytoprotective gene heme oxygenase 1 (HO-1) could be induced by nuclear factor E2-related factor 2 (Nrf2) nuclear translocation. The purpose of this study was to determine the role of Brahma-related gene 1 (Brg1), a catalytic subunit of SWI2/SNF2-like chromatin remodeling complexes, in Nrf2/HO-1 pathway activation during hepatic ischemia-reperfusion (HIR). Our results showed that hepatic Brg1 was inhibited during early HIR while Brg1 overexpression reduced oxidative injury in CMV-Brg1 mice subjected to HIR. Moreover, promoter-driven luciferase assay showed that overexpression of Brg1 by adenovirus transfection in AML12 cells selectively enhanced HO-1 gene expression after hypoxia/reoxygenation (H/R) treatment but did not affect the other Nrf2 target gene NQO1. Furthermore, inhibition of HO-1 by the selective HO-1 inhibitor zinc protoporphyria could partly reverse the hepatic protective effects of Brg1 overexpression while HO-1-Adv attenuated AML12 cells H/R damage. Further, chromatin immunoprecipitation analysis revealed that Brg1 overexpression, which could significantly increase the recruitment of Brg1 protein to HO-1 but not NQO1 promoter, was recruited by Nrf2 to the HO-1 regulatory regions in AML12 hepatocytes subjected to H/R. In conclusion, our results demonstrated that restoration of Brg1 during reperfusion could enhance Nrf2-mediated inducible expression of HO-1 during HIR to effectively increase antioxidant ability to combat against hepatocytes damage.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Active Transport, Cell Nucleus / drug effects
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Adenoviridae / genetics
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Adenoviridae / metabolism
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Animals
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Cell Line
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Chromatin / chemistry
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Chromatin / metabolism
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DNA Helicases / genetics*
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DNA Helicases / metabolism
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Gene Expression Regulation
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Genes, Reporter
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Genetic Vectors / chemistry
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Genetic Vectors / metabolism
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Heme Oxygenase-1 / antagonists & inhibitors
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Heme Oxygenase-1 / genetics*
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Heme Oxygenase-1 / metabolism
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Hepatocytes / metabolism*
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Hepatocytes / pathology
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Liver / metabolism*
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Liver / pathology
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Luciferases / genetics
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Luciferases / metabolism
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Membrane Proteins / antagonists & inhibitors
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Membrane Proteins / genetics*
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Membrane Proteins / metabolism
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Mice
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Mice, Transgenic
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NAD(P)H Dehydrogenase (Quinone) / genetics
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NAD(P)H Dehydrogenase (Quinone) / metabolism
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NF-E2-Related Factor 2 / genetics*
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NF-E2-Related Factor 2 / metabolism
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Nuclear Proteins / genetics*
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Nuclear Proteins / metabolism
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Promoter Regions, Genetic
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Protein Transport
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Protoporphyrins / pharmacology
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Reperfusion Injury / genetics*
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Reperfusion Injury / metabolism
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Reperfusion Injury / pathology
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Signal Transduction
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Transcription Factors / genetics*
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Transcription Factors / metabolism
Substances
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Chromatin
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Membrane Proteins
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NF-E2-Related Factor 2
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Nfe2l2 protein, mouse
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Nuclear Proteins
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Protoporphyrins
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Transcription Factors
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zinc protoporphyrin
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Luciferases
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Heme Oxygenase-1
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Hmox1 protein, mouse
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NAD(P)H Dehydrogenase (Quinone)
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NQO1 protein, human
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Smarca4 protein, mouse
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DNA Helicases