A duplex endpoint PCR assay for rapid detection and differentiation of Leptospira strains

Rev Soc Bras Med Trop. 2017 Mar-Apr;50(2):239-242. doi: 10.1590/0037-8682-0364-2016.

Abstract

Introduction:: This study aimed to develop a duplex endpoint PCR assay for rapid detection and differentiation of Leptospira strains.

Methods:: Primers were designed to target the rrs (LG1/LG2) and ligB (LP1/LP2) genes to confirm the presence of the Leptospira genus and the pathogenic species, respectively.

Results:: The assay showed 100% specificity against 17 Leptospira strains with a limit of detection of 23.1pg/µl of leptospiral DNA and sensitivity of 103 leptospires/ml in both spiked urine and water.

Conclusions:: Our duplex endpoint PCR assay is suitable for rapid early detection of Leptospira with high sensitivity and specificity.

MeSH terms

  • DNA Primers*
  • DNA, Bacterial* / isolation & purification
  • Leptospira / classification*
  • Leptospira / genetics
  • Leptospira / isolation & purification
  • Polymerase Chain Reaction / methods*
  • Sensitivity and Specificity
  • Species Specificity

Substances

  • DNA Primers
  • DNA, Bacterial