A wash-free SNAP-tag fluorogenic probe based on the additive effects of quencher release and environmental sensitivity

Shuang Leng; Qinglong Qiao; Lu Miao; Wuguo Deng; Jingnan Cui; Zhaochao Xu

doi:10.1039/c7cc01483j

A wash-free SNAP-tag fluorogenic probe based on the additive effects of quencher release and environmental sensitivity

Chem Commun (Camb). 2017 Jun 13;53(48):6448-6451. doi: 10.1039/c7cc01483j.

Authors

Shuang Leng¹, Qinglong Qiao, Lu Miao, Wuguo Deng, Jingnan Cui, Zhaochao Xu

Affiliation

¹ Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China. zcxu@dicp.ac.cn.

PMID: 28560360
DOI: 10.1039/c7cc01483j

Abstract

A 1,8-naphthalimide-derived fluorogenic probe was reported to label SNAP-tag fusion proteins in living cells. The probe can rapidly label a SNAP-tag and exhibit a fluorescence increase of 36-fold due to the additive effects of environment sensitivity of fluorophores and inhibition of photo-induced electron transfer from O⁶-benzylguanine to the fluorophore. The labeling of intracellular proteins has been successfully achieved without a wash-out procedure.