Evaluating the non-lethal effects of organophosphorous and carbamate insecticides on the yabby (Cherax destructor) using cholinesterase (AChE, BChE), Glutathione S-Transferase and ATPase as biomarkers

Abstract

The toxicity of two organophosphorus insecticides, chlorpyrifos (CPF), malathion (MAL), and one carbamate insecticide, methomyl (METH), to the yabby (Cherax destructor) was assessed by measuring cholinesterase (AChE, BChE), Glutathione S-Transferase (GST) and Na⁺/K⁺ATPase activity after 96h of exposure. Yabbies exposed to all three insecticides at 2 and 5µgL^-1 exhibited significant AChE, BChE, GST and Na⁺/K⁺ATPase inhibition. Based on these enzyme inhibition tests, the toxicity of the three insecticides to C. destructor was CPF > MAL > METH. After 14 days of recovery the yabbies enzymatic activities of AChE, BChE, GST and Na⁺/K⁺ATPase was measured. Recovery of The enzyme activity recovery was faster after the exposure to METH than for the yabbies exposed to CPF and MAL. Slow recovery of enzyme activity could affect the physical activities of organisms and produce indirect effects on populations if such crayfish are less able to elude predators or search for food.

Keywords: Acetylcholinesterase; Butyrylcholinesterase; Cherax destructor; Chlorpyrifos; Glutathione S-Transferase; Malathion; Methomyl; Na+/K+ATPase.