Structural studies of the O-specific polysaccharide from detergent degrading bacteria Pseudomonas putida TSh-18

Abstract

An O-specific polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of bacteria Pseudomonas putida TSh-18, capable of degrading non-ionogenic technical detergents. The polysaccharide was found to contain a rarely occurring sugar derivative 4,6-dideoxy-4-[(R)-3-hydroxybutanoylamino]-d-galactose [d-Fucp4N(RHb)]. Sugar and methylation analyses, Smith degradation, solvolysis with CF₃CO₂H, and ¹H and ¹³C NMR spectroscopy enabled elucidation of the following structure of the branched trisaccharide repeating units of the polysaccharide.

Keywords: 2,4-Diacetamido-2,4,6-trideoxy-d-glucose; 4,6-Dideoxy-4-[(R)-3-hydroxybutanoylamino]-d-galactose; Bacterial polysaccharide structure; Lipopolysaccharide; Pseudomonas putida.