Scanning Electron Microscopy Coupled with Energy Dispersive Spectrometric Analysis Reveals for the First Time Weddellite and Sylvite Crystals on the Surface of Involucral Bracts and Petals of two Xeranthemum L. (Compositae) Species

Microsc Microanal. 2017 Jun;23(3):679-686. doi: 10.1017/S1431927617000551.

Abstract

In this work, weddellite and sylvite crystals are identified for the first time on the involucral bracts and petals of Xeranthemum annuum and Xeranthemum cylindraceum using scanning electron microscopy coupled with energy dispersive spectrometric (SEM-EDS) analysis. Well-developed crystals of weddellite (CaC2O4·2H2O) occur in the form of a tetragonal bipyramid (hhl), rarely in combination of a bipyramid and tetragonal prism (h00). Indumentum of involucral bracts of X. cylindraceum consists of nonglandular and glandular trichomes. Sylvite (KCl) crystals are observed only on the petal surface of X. cylindraceum. The crystals of sylvite occur in the form of perfect cubes (hexahedrons), but some crystals are deformed, i.e., partially elongated. Taxonomic significance of investigated microcharacters as well as the use of SEM-EDS analysis in taxonomic studies of plants are discussed.

Keywords: Xeranthemum; Cardueae; SEM-EDS; involucral bracts and petals; weddellite and sylvite crystals.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Asteraceae / chemistry*
  • Asteraceae / classification
  • Asteraceae / metabolism
  • Asteraceae / ultrastructure*
  • Calcium / metabolism
  • Calcium Oxalate / chemistry*
  • Crystallization
  • Cytological Techniques / methods*
  • Flowers / chemistry
  • Flowers / ultrastructure
  • Microscopy, Electron, Scanning / methods*
  • Phytochemicals / chemistry
  • Potassium / metabolism
  • Potassium Chloride / chemistry*
  • Serbia
  • Spectrometry, X-Ray Emission / methods*

Substances

  • Phytochemicals
  • Calcium Oxalate
  • Potassium Chloride
  • weddellite
  • Potassium
  • Calcium