Characterization of a newly isolated freshwater Eustigmatophyte alga capable of utilizing far-red light as its sole light source

Photosynth Res. 2018 Mar;135(1-3):177-189. doi: 10.1007/s11120-017-0401-z. Epub 2017 May 25.

Abstract

Oxygenic phototrophs typically utilize visible light (400-700 nm) to drive photosynthesis. However, a large fraction of the energy in sunlight is contained in the far-red region, which encompasses light beyond 700 nm. In nature, certain niche environments contain high levels of this far-red light due to filtering by other phototrophs, and in these environments, organisms with photosynthetic antenna systems adapted to absorbing far-red light are able to thrive. We used selective far-red light conditions to isolate such organisms in environmental samples. One cultured organism, the Eustigmatophyte alga Forest Park Isolate 5 (FP5), is able to absorb far-red light using a chlorophyll (Chl) a-containing antenna complex, and is able to grow under solely far-red light. Here we characterize the antenna system from this organism, which is able to shift the absorption of Chl a to >705 nm.

Keywords: Antenna; Eustigmatophyte; Far-red light; Light harvesting complex; Stramenopila.

MeSH terms

  • Chromatography, High Pressure Liquid
  • Electrophoresis, Gel, Two-Dimensional
  • Fresh Water*
  • Light*
  • Multiprotein Complexes / isolation & purification
  • Phylogeny
  • Pigments, Biological / metabolism
  • Plant Proteins / isolation & purification
  • Plants / radiation effects*
  • Plants / ultrastructure
  • Spectrometry, Fluorescence

Substances

  • Multiprotein Complexes
  • Pigments, Biological
  • Plant Proteins