Next-Generation Nucleic Acid Aptamers with Two-Base-Modified Nucleotides Have Improved Binding Affinity and Potency

Hadi AlShamaileh; Rakesh N Veedu

doi:10.1002/cbic.201700276

Next-Generation Nucleic Acid Aptamers with Two-Base-Modified Nucleotides Have Improved Binding Affinity and Potency

Chembiochem. 2017 Aug 17;18(16):1565-1567. doi: 10.1002/cbic.201700276. Epub 2017 Jun 26.

Authors

Hadi AlShamaileh¹, Rakesh N Veedu^{1

2}

Affiliations

¹ Centre for Comparative Genomics, Murdoch University, Murdoch, Perth, 6150, Australia.
² Perron Institute for Neurological and Translational Science, Nedlands, Perth, 6009, Australia.

PMID: 28544018
DOI: 10.1002/cbic.201700276

Abstract

Twice as apt: Nucleic acid aptamers with high binding affinity, specificity, epitope coverage and nuclease resistance were developed by using libraries containing oligonucleotides in which two bases in the pyrimidine nucleotide had been modified.

Keywords: PCSK9; SELEX; SOMAmer; aptamers; nucleotides.

MeSH terms

Animals
Aptamers, Nucleotide / chemical synthesis
Aptamers, Nucleotide / chemistry*
Drug Design
Haplorhini
Humans
Lipoproteins, LDL / metabolism
Mice
PCSK9 Inhibitors
Proprotein Convertase 9 / blood
Proprotein Convertase 9 / chemistry*
Pyrimidine Nucleotides / chemical synthesis
Pyrimidine Nucleotides / chemistry*
Rats

Substances

Aptamers, Nucleotide
Lipoproteins, LDL
PCSK9 Inhibitors
Pyrimidine Nucleotides
PCSK9 protein, human
PCSK9 protein, rat
Pcsk9 protein, mouse
Proprotein Convertase 9