Improving of lipid productivity of the oleaginous microalgae Chlorella pyrenoidosa via atmospheric and room temperature plasma (ARTP)

Song Cao; Xu Zhou; Wenbiao Jin; Feng Wang; Renjie Tu; Songfang Han; Hongyi Chen; Chuan Chen; Guo-Jun Xie; Fang Ma

doi:10.1016/j.biortech.2017.05.039

Improving of lipid productivity of the oleaginous microalgae Chlorella pyrenoidosa via atmospheric and room temperature plasma (ARTP)

Bioresour Technol. 2017 Nov;244(Pt 2):1400-1406. doi: 10.1016/j.biortech.2017.05.039. Epub 2017 May 10.

Authors

Song Cao¹, Xu Zhou¹, Wenbiao Jin¹, Feng Wang¹, Renjie Tu¹, Songfang Han¹, Hongyi Chen¹, Chuan Chen², Guo-Jun Xie², Fang Ma³

Affiliations

¹ Harbin Institute of Technology Shenzhen Graduate School, 518055 Shenzhen, China; Shenzhen Engineering Laboratory of Microalgal Bioenergy, Harbin Institute of Technology, 518055 Shenzhen, China.
² State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, 150090 Harbin, China.
³ State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, 150090 Harbin, China. Electronic address: mafang@hit.edu.cn.

PMID: 28539241
DOI: 10.1016/j.biortech.2017.05.039

Abstract

In this study, an efficient screening program was established with ARTP. Five strains from oleaginous microalgae Chlorella pyrenoidosa were screened from mutant library after mutagenizing by ARTP. Among them, the optimal mutant strain was named as II-H6. In the BG11 medium, the OD₆₈₀ of II-H6 in stationary phase were increased by 32.08% than the original strain. Meanwhile, compared with the original strain, the dry weight and lipid productivity of II-H6 were increased by 22.07% and 16.85%, respectively. II-H6 showed a good genetic stability in BG11 medium and the optimum growth temperature and pH were 33°C and 9.0. 18S gene fragment length of II-H6 strain were 1886bp. Analysis of the gene fragment showed that the II-H6 strain had a close relationship to the original strain, and it belonged to the mutation within the genus Chlorella.

Keywords: Atmospheric and room temperature plasma (ARTP); Biodiesel; Biomass; Chlorella pyrenoidosa; Mutation.

MeSH terms

Biomass
Chlorella*
Lipids*
Microalgae
Temperature

Substances

Lipids