Hepatic anti-inflammatory effect of hexane extracts of Dioscorea batatas Decne: Possible suppression of toll-like receptor 4-mediated signaling

Biomed Pharmacother. 2017 Aug:92:157-167. doi: 10.1016/j.biopha.2017.05.036. Epub 2017 May 21.

Abstract

The hepatic anti-inflammatory potential of hexane extracts of Dioscorea batatas Decne edible part (EDH-1e) and bark (EDH-2b) were investigated in Western-type diet-fed apolipoprotein E null [ApoE (-/-)] mice and HepG2 cells. EDH-1e and EDH-2b suppressed the increased levels of tumor necrosis factor alpha (TNF-α), interleukin (IL)-6, transforming growth factor beta 1 (TGF-β1), vascular cell adhesion protein 1 (VCAM-1), and monocyte chemoattractant protein-1 (MCP-1), and reduced infiltration of monocytes into liver tissue. The protein levels of Toll-like receptor 4 (TLR4) were also downregulated by EDH-1e and EDH-2b treatment as were the levels of activator protein 1 (AP-1), c-fos, and c-jun in the livers from Western-type diet-fed ApoE (-/-) mice and in lipopolysaccharide-stimulated HepG2 cells. Taken together, EDH-1e and EDH-2b attenuated hepatic inflammation and fibrosis via suppression of the TLR4-AP1-mediated signaling pathway.

Keywords: Activator protein 1; ApoE; Dioscorea batatas Decne; HepG2; Inflammation; Toll-like receptor 4.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Anti-Inflammatory Agents, Non-Steroidal / therapeutic use*
  • Diet, Western / adverse effects
  • Dioscorea / chemistry*
  • Ethnopharmacology
  • Hep G2 Cells
  • Hepatocytes / drug effects*
  • Hepatocytes / immunology
  • Hepatocytes / metabolism
  • Hepatocytes / pathology
  • Hexanes / chemistry
  • Humans
  • Male
  • Medicine, Korean Traditional
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Monocytes / drug effects
  • Monocytes / immunology
  • Monocytes / metabolism
  • Monocytes / pathology
  • Non-alcoholic Fatty Liver Disease / drug therapy*
  • Non-alcoholic Fatty Liver Disease / immunology
  • Non-alcoholic Fatty Liver Disease / metabolism
  • Non-alcoholic Fatty Liver Disease / pathology
  • Plant Bark / chemistry
  • Plant Extracts / therapeutic use*
  • Rhizome / chemistry
  • Signal Transduction / drug effects*
  • Solvents / chemistry
  • Toll-Like Receptor 4 / antagonists & inhibitors*
  • Toll-Like Receptor 4 / metabolism
  • Transcription Factor AP-1 / antagonists & inhibitors
  • Transcription Factor AP-1 / metabolism

Substances

  • Anti-Inflammatory Agents, Non-Steroidal
  • Hexanes
  • Plant Extracts
  • Solvents
  • Toll-Like Receptor 4
  • Transcription Factor AP-1