Abstract
A convergent strategy was developed for the first-time synthesis of biotin-labeled GPI core glycans. These GPI conjugates are useful for various biological studies showcased by their application in the scrutiny of pore-forming bacterial toxin-GPI interaction, revealing that the phosphate group at the GPI inositol 1-O-position had a significant impact on GPI-toxin binding.
MeSH terms
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Anisoles / chemistry
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Bacterial Proteins / chemistry
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Bacterial Proteins / metabolism
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Bacterial Toxins / analysis
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Bacterial Toxins / metabolism*
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Biotin / chemistry*
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Biotin / metabolism
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Cerium / chemistry
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Enzyme-Linked Immunosorbent Assay
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Glycosylphosphatidylinositols / chemistry
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Glycosylphosphatidylinositols / metabolism*
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Hemolysin Proteins / chemistry
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Hemolysin Proteins / metabolism
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Streptococcus agalactiae / metabolism
Substances
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Anisoles
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Bacterial Proteins
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Bacterial Toxins
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CAMP protein, Streptococcus
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Glycosylphosphatidylinositols
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Hemolysin Proteins
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Cerium
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Biotin
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anisole
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ceric ammonium nitrate