The first report of CD2 associated protein gene, in a teleost (Rock bream, Oplegnathus fasciatus): An investigation of the immune response upon infection with several pathogens

Fish Shellfish Immunol. 2017 Aug:67:1-6. doi: 10.1016/j.fsi.2017.05.045. Epub 2017 May 20.

Abstract

CD2 is expressed on the surfaces of virtually all T cells and natural killer (NK) cells. In mammals, the CD2 molecule is 50 kDa. The cytoplasmic tail of CD2 interacts with CD2-associated protein (CD2AP), which plays an important role in mediating the trigger signal in outer magnetic pole cells. In this study, we identified CD2AP from rock bream and investigated its gene expression. The ORF of CD2AP (1950 bp) encodes 650 amino acids (aa). CD2AP has a Src homology 3 (SH3) domain. Quantitative real-time PCR analysis revealed that CD2AP shows higher expression in the gills and skin. Under experimental challenge, CD2AP gene expression was increased as relative to the control after 7 days. This result will improve our understanding of blood vessels in teleost fish, and will provide a basis for the study of CD2-related genes.

Keywords: CD2-Associated protein; Gene expression; Rock bream; SH3 domain; T cells.

MeSH terms

  • Adaptor Proteins, Signal Transducing / chemistry
  • Adaptor Proteins, Signal Transducing / genetics*
  • Adaptor Proteins, Signal Transducing / metabolism
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cytoskeletal Proteins / chemistry
  • Cytoskeletal Proteins / genetics*
  • Cytoskeletal Proteins / metabolism
  • DNA Virus Infections / genetics
  • DNA Virus Infections / immunology
  • DNA Virus Infections / veterinary
  • DNA, Complementary / genetics
  • DNA, Complementary / metabolism
  • Edwardsiella tarda / physiology
  • Enterobacteriaceae Infections / genetics
  • Enterobacteriaceae Infections / immunology
  • Enterobacteriaceae Infections / microbiology
  • Enterobacteriaceae Infections / veterinary
  • Fish Diseases / genetics*
  • Fish Diseases / immunology
  • Fish Diseases / microbiology
  • Fish Diseases / virology
  • Fish Proteins / chemistry
  • Fish Proteins / genetics*
  • Fish Proteins / metabolism
  • Gene Expression Profiling
  • Immunity, Innate*
  • Iridoviridae / physiology
  • Organ Specificity
  • Perciformes*
  • Phylogeny
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Sequence Alignment / veterinary
  • Streptococcal Infections / genetics
  • Streptococcal Infections / immunology
  • Streptococcal Infections / microbiology
  • Streptococcal Infections / veterinary
  • Streptococcus iniae / physiology

Substances

  • Adaptor Proteins, Signal Transducing
  • CD2-associated protein
  • Cytoskeletal Proteins
  • DNA, Complementary
  • Fish Proteins
  • RNA, Messenger