Co-overexpression of response regulator genes slr1037 and sll0039 improves tolerance of Synechocystis sp. PCC 6803 to 1-butanol

Xinyan Gao; Tao Sun; Lina Wu; Lei Chen; Weiwen Zhang

doi:10.1016/j.biortech.2017.04.112

Co-overexpression of response regulator genes slr1037 and sll0039 improves tolerance of Synechocystis sp. PCC 6803 to 1-butanol

Bioresour Technol. 2017 Dec;245(Pt B):1476-1483. doi: 10.1016/j.biortech.2017.04.112. Epub 2017 May 4.

Authors

Xinyan Gao¹, Tao Sun¹, Lina Wu¹, Lei Chen², Weiwen Zhang³

Affiliations

¹ Laboratory of Synthetic Microbiology, School of Chemical Engineering & Technology, Tianjin University, Tianjin, PR China; Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, Tianjin, PR China; SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering, Tianjin, PR China.
² Laboratory of Synthetic Microbiology, School of Chemical Engineering & Technology, Tianjin University, Tianjin, PR China; Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, Tianjin, PR China; SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering, Tianjin, PR China. Electronic address: lchen@tju.edu.cn.
³ Laboratory of Synthetic Microbiology, School of Chemical Engineering & Technology, Tianjin University, Tianjin, PR China; Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, Tianjin, PR China; SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering, Tianjin, PR China; Center for Biosafety Research and Strategy, Tianjin University, Tianjin, PR China.

PMID: 28533065
DOI: 10.1016/j.biortech.2017.04.112

Abstract

In this study, two response regulator (RR) encoding genes slr1037 as well as sll0039 were co-overexpressed in Synechocystis sp. PCC 6803 by metabolic engineering and the 1-butanol tolerance was successfully improved by 133%. Aiming to explore the possible mechanisms for the enhancing 1-butanol tolerance, a quantitative iTRAQ-LC-MS/MS proteomics approach was then employed, identifying 216 up-regulated and 99 down-regulated proteins compared to wild type after 1-butanol treatment. This study mapped the potential target genes regulated by Slr1037 and Sll0039 and demonstrated the feasibility of engineering response regulators for modifying the biofuel tolerance in cyanobacteria.

Keywords: 1-Butanol tolerance; Co-overexpression; Response regulators; Synechocystis.

MeSH terms

1-Butanol*
Bacterial Proteins / metabolism
Butanols
Drug Tolerance
Gene Expression Regulation, Bacterial*
Proteomics
Synechocystis*

Substances

Bacterial Proteins
Butanols
1-Butanol