Abstract
Two new pterosin glycosides, (2S,3S)-pterosin C 3-O-β-d-(4'-(E)-caffeoyl)-glucopyranoside (1) and (2S,3S)-pterosin C 3-O-β-d-(6'-(E)-p-coumaroyl)-glucopyranoside (2), were isolated from Pteris multifida (Pteridaceae) roots along with ten known pterosin compounds (3-12). The chemical structures of the isolated compounds were elucidated by extensive analysis of the 1D, 2D NMR, HRESIMS, and CD spectroscopic data. The cytotoxicities of 1-12 against HCT116 human colorectal cancer cell line were evaluated. Among the isolates, compound 1 showed moderate antiproliferative activity in HCT116 cells with an IC50 value of 8.0±1.7μM. Additionally, 1 induced the upregulation of the caspase-9 and procaspase-9 levels in Western blots and increased the annexin V/propidium iodide (PI)-positive cell population in flow cytometry.
Keywords:
Cytotoxicity; HCT116 cells; Pteris multifida; Pterosin.
Copyright © 2017 Elsevier Ltd. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antineoplastic Agents, Phytogenic / chemistry
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Antineoplastic Agents, Phytogenic / isolation & purification
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Antineoplastic Agents, Phytogenic / toxicity
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Caspase 9 / metabolism
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Cell Proliferation / drug effects
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Circular Dichroism
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Colonic Neoplasms
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HCT116 Cells
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Humans
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Indans / chemistry*
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Indans / isolation & purification
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Indans / pharmacology*
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Magnetic Resonance Spectroscopy
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Mass Spectrometry
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Molecular Conformation
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Plant Extracts / chemistry
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Plant Roots / chemistry
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Plant Roots / metabolism
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Pteris / chemistry*
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Pteris / metabolism
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Sesquiterpenes / chemistry*
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Sesquiterpenes / isolation & purification
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Sesquiterpenes / pharmacology*
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Sesquiterpenes / toxicity
Substances
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(2S,3S)-pterosin C 3-O-beta-D-(40-(E)-caffeoyl)-glucopyranoside
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(2S,3S)-pterosin C 3-O-beta-D-(6'-(E)-p-coumaroyl)-glucopyranoside
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Antineoplastic Agents, Phytogenic
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Indans
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Plant Extracts
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Sesquiterpenes
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pterosin
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Caspase 9