Determination of volumetric gas-liquid mass transfer coefficient of carbon monoxide in a batch cultivation system using kinetic simulations

Abstract

A mathematical model of microbial kinetics was introduced to predict the overall volumetric gas-liquid mass transfer coefficient (k_La) of carbon monoxide (CO) in a batch cultivation system. The cell concentration (X), acetate concentration (C_ace), headspace gas (N_co and [Formula: see text] ), dissolved CO concentration in the fermentation medium (C_co), and mass transfer rate (R) were simulated using a variety of k_La values. The simulated results showed excellent agreement with the experimental data for a k_La of 13/hr. The C_co values decreased with increase in cultivation times, whereas the maximum mass transfer rate was achieved at the mid-log phase due to vigorous microbial CO consumption rate higher than R. The model suggested in this study may be applied to a variety of microbial systems involving gaseous substrates.

Keywords: Batch cultivation; Carbon monoxide; Eubacterium limosum KIST612; Gas–liquid mass transfer; Kinetic simulation.