Objectives: To improve heterologous proteins production, we constructed a maltose-inducible expression system in Bacillus subtilis.
Results: An expression system based on the promoter for maltose utilization constructed in B. subtilis. Successively, to improve the performance of the P malA -derived system, mutagenesis was employed by gradually shortening the length of P malA promoter and altering the spacing between the predicted MalR binding site and the -35 region. Furthermore, deletion of the maltose utilization genes (malL and yvdK) improved the P malA promoter activity. Finally, using this efficient maltose-inducible expression system, we enhanced the production of luciferase and D-aminoacylase, compared with the P hpaII system.
Conclusions: A maltose-inducible expression system was constructed and evaluated. It could be used for high level expression of heterologous proteins production.
Keywords: Bacillus subtilis; D-Aminoacylase; Expression vector; Gene deletion; Luciferase; P malA promoter.