MALDI-MSI of Lipids in Human Skin

Philippa J Hart; Malcolm R Clench

doi:10.1007/978-1-4939-7051-3_4

MALDI-MSI of Lipids in Human Skin

Methods Mol Biol. 2017:1618:29-36. doi: 10.1007/978-1-4939-7051-3_4.

Authors

Philippa J Hart¹, Malcolm R Clench²

Affiliations

¹ Waters Corporation, Stamford Ave., Altrincham Road, Wilmslow, SK9 4AX, UK. Philippa_hart@waters.com.
² Centre for Mass Spectrometry Imaging, Biomolecular Sciences Research Centre, Sheffield Hallam University, Sheffield, UK.

PMID: 28523497
DOI: 10.1007/978-1-4939-7051-3_4

Abstract

Matrix-assisted laser desorption ionization (MALDI) mass spectrometry (MS) is now a well-established technique for imaging analysis of sectioned biological tissues. One of the growing areas of interest is in the analysis of skin. MALDI-MSI can provide a wealth of information from within sections of skin. This includes information on the distribution of pharmaceuticals following topical treatments, through to the examination of the composition of different skin layers and studies of proteomic, lipidomic, and metabolomic responses to disease, wounds, and external stimuli. Here, we describe the handling procedures, preparatory treatment, and mass spectrometry setup required for the MALDI MSI analysis of lipids within human skin samples.

Keywords: Imaging; Lipids; MALDI; MS; MSI; Skin.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Humans
Lipids / analysis*
Proteomics / methods
Skin / metabolism*
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*

Substances

Lipids