Objective: To investigate the influence of aluminum chloride (AlCl(3)) solution on the embryon-ic development of zebrafish and neurobehavior of juvenile fish. Methods: The embryos of zebrafishat 6 hours after fertilization were exposed to AlCl(3) solution at a concentration of 0, 55.0, 60.5, 66.6, 73.5, 80.5, or 100.0 mg/L, and embryonic hatching rates at 48 and 72 hours after fertilization were calculated. The embryos of zebrafishat 6 hours after fertilization were exposed to AlCl(3) solution at a concentration of 0, 60.0, 72.0, 86.4, 103.7, or 124.4 mg/L, and the embryonic mortality rates at 12, 24, 48, 72, and 96 hours after fertilization were calculat-ed. The embryos of zebrafish at 6 hours after fertilization were exposed to AlCl(3) solution at a concentration of 0, 50, 100, 200, 400, or 800 μg/L, and the changes in the neurobehavior of juvenile fish were observed after hatching, including touch-escape reaction at 72 hours after fertilization and autonomic movement and panic es-cape reflex at 7 days after fertilization. Results: Compared with the 0 mg/L group, the≥66.6 mg/L group had a sig-nificant reduction in embryonic hatching rate at 48 and 72 hours after fertilization, and the ≥72.0 mg/L group had a significant increase in embryonic mortality rate at 96 hours after fertilization (P<0.05) . Compared with the 0 μg/L group, the≥100 μg/L group had a significant reduction in the number of times of touch-escape reaction (P<0.05) .Compared with the 0 and 50 μg/L groups, the 100-800 μg/L groups had significant reductions in total movement distance and average speed (P<0.05) . Compared with the dark period before illumination, all groups had a significant increase in movement speed during the light period of the panic escape reflex test (i.e., the third minute) (P<0.05) ; within 2 minutes after the light was turned off, there was no significant change in movement speed in the 0-200 μg/L groups (P>0.05) ; the 400 and 800 μg/L groups had a significant increase in movement speed (P<0.05) . Conclusion: AlCl(3) exposure may cause embryonic developmental disorder in zebrafish and ab-normal neurobehavior in juvenile fish.
目的： 探讨氯化铝（AlCl(3)）溶液对斑马鱼胚胎和幼鱼神经行为的影响。 方法： 将受精后6 h的斑马鱼胚胎分别暴露于0、55.0、60.5、66.6、73.5、80.5和100.0 mg/L AlCl(3)溶液中，分别计算受精后48、72 h的斑马鱼胚胎孵化率；将受精后6 h的斑马鱼胚胎分别暴露于0、60.0、72.0、86.4、103.7和124.4 mg/L AlCl(3)溶液中，分别计算受精后12、24、48、72和96 h的斑马鱼胚胎死亡率；将受精后6 h的斑马鱼胚胎分别暴露于0、50、100、200、400和800 μg/L AlCl(3)溶液中，待其孵化后观察幼鱼的神经行为变化，包括受精后72 h的幼鱼触动逃避反应、受精后7 d的幼鱼自发运动以及惊恐逃避反射。 结果： 与0 mg/L浓度组相比，≥66.6 mg/L浓度组斑马鱼受精后48、72 h胚胎孵化率明显下降，≥72.0 mg/L浓度组斑马鱼受精后96 h的胚胎死亡率明显增加，差异均有统计学意义（P<0.05）。与0 μg/L浓度组相比，≥100 μg/L浓度组斑马鱼幼鱼触动逃避次数明显减少，差异均有统计学意义（P<0.05）。与0和50 μg/L浓度组相比，≥100 μg/L浓度组斑马鱼总运动距离和平均速度均明显降低，差异均有统计学意义（P<0.05）。与光照前黑暗期相比，受精后7 d各浓度组斑马鱼幼鱼惊恐逃避反射试验光照期（即第3分钟）运动速度均加快（P<0.05）；在关闭光源后2 min内，100和200 μg/L浓度组幼鱼运动速度与光照前黑暗期相比，差异无统计学意义（P>0.05）；400和800 μg/L浓度组幼鱼运动速度明显高于光照前水平，差异均有统计学意义（P<0.05）。 结论： AlCl(3)暴露可能导致斑马鱼胚胎发育障碍以及幼鱼神经行为异常。.
Keywords: Aluminium chloride; Developmental neurotoxicity; Embryos; Zebrafish.