Analysis of Human Papillomavirus Genome Replication Using Two- and Three-Dimensional Agarose Gel Electrophoresis

Liisi Henno; Eva-Maria Tombak; Jelizaveta Geimanen; Marit Orav; Ene Ustav; Mart Ustav

doi:10.1002/cpmc.28

Analysis of Human Papillomavirus Genome Replication Using Two- and Three-Dimensional Agarose Gel Electrophoresis

Curr Protoc Microbiol. 2017 May 16:45:14B.10.1-14B.10.37. doi: 10.1002/cpmc.28.

Authors

Liisi Henno¹, Eva-Maria Tombak¹, Jelizaveta Geimanen¹, Marit Orav¹, Ene Ustav¹, Mart Ustav^{1

2}

Affiliations

¹ University of Tartu, Institute of Technology, Tartu, Estonia.
² Academy of Sciences, Tallinn, Estonia.

PMID: 28510360
DOI: 10.1002/cpmc.28

Abstract

This unit includes the necessary information to conduct neutral/neutral and neutral/alkaline two-dimensional and neutral/neutral/alkaline three-dimensional agarose gel electrophoresis. The methodology has been optimized over the years to gain a better outcome from the hard-to-interpret signals of human papilloma virus replication intermediates obtained from two- and three-dimensional agarose gels. Examples of typical results and interpretation of replication intermediate patterns are included, and the outcomes of multiple-dimension assays are assessed using previously published experimental data. © 2017 by John Wiley & Sons, Inc.

Keywords: DNA replication; HPV; minicircle production; southern blot; three-dimensional agarose gel electrophoresis; two-dimensional agarose gel electrophoresis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

DNA, Viral / analysis
Electrophoresis, Agar Gel / methods*
Humans
Papillomaviridae / physiology*
Virology / methods*
Virus Replication*

Substances

DNA, Viral