Anti-carbamylated Protein Antibodies Are Detectable in Various Connective Tissue Diseases

Shuichiro Nakabo; Hajime Yoshifuji; Motomu Hashimoto; Yoshitaka Imura; Ran Nakashima; Kosaku Murakami; Nobuo Kuramoto; Shinji Ito; Junko Satoh; Masao Tanaka; Takao Fujii; Tsuneyo Mimori; Koichiro Ohmura

doi:10.3899/jrheum.161432

Anti-carbamylated Protein Antibodies Are Detectable in Various Connective Tissue Diseases

J Rheumatol. 2017 Sep;44(9):1384-1388. doi: 10.3899/jrheum.161432. Epub 2017 May 15.

Authors

Shuichiro Nakabo^{1

2}, Hajime Yoshifuji^{1

2}, Motomu Hashimoto^{1

2}, Yoshitaka Imura^{1

2}, Ran Nakashima^{1

2}, Kosaku Murakami^{1

2}, Nobuo Kuramoto^{1

2}, Shinji Ito^{1

2}, Junko Satoh^{1

2}, Masao Tanaka^{1

2}, Takao Fujii^{1

2}, Tsuneyo Mimori^{1

2}, Koichiro Ohmura^{3

4}

Affiliations

¹ From the Department of Rheumatology and Clinical Immunology, Graduate School of Medicine, Kyoto University, Kyoto; Department of Clinical Immunology and Rheumatology, Wakayama Medical University, Wakayama, Japan.
² S. Nakabo, MD, research fellow, Department of Rheumatology and Clinical Immunology, Graduate School of Medicine, Kyoto University; H. Yoshifuji, MD, PhD, Assistant professor, Department of Rheumatology and Clinical Immunology, Graduate School of Medicine, Kyoto University; M. Hashimoto, MD, PhD, Assistant professor, Department of the Control for Rheumatic Diseases, Graduate School of Medicine, Kyoto University; Y. Imura, MD, PhD, Assistant professor, Department of Rheumatology and Clinical Immunology, Graduate School of Medicine, Kyoto University; R. Nakashima, MD, PhD, Assistant professor, Department of Rheumatology and Clinical Immunology, Graduate School of Medicine, Kyoto University; K. Murakami, MD, PhD, Assistant professor, Department of Rheumatology and Clinical Immunology, Graduate School of Medicine, Kyoto University; N. Kuramoto, MD, PhD, Assistant professor, Department of Rheumatology and Clinical Immunology, Graduate School of Medicine, Kyoto University; S. Ito, PhD, Assistant professor, Medical Research Support Center, Graduate School of Medicine, Kyoto University; J. Satoh, PhD, Postdoctoral fellow, Medical Research Support Center, Graduate School of Medicine, Kyoto University; M. Tanaka, MD, PhD, Associate professor, Department of the Control for Rheumatic Diseases, Graduate School of Medicine, Kyoto University; T. Fujii, MD, PhD, Professor, Department of Clinical Immunology and Rheumatology, Wakayama Medical University, and Department of Rheumatology and Clinical Immunology, Graduate School of Medicine, Kyoto University; T. Mimori, MD, PhD, Professor, Department of Rheumatology and Clinical Immunology, Graduate School of Medicine, Kyoto University; K. Ohmura, MD, PhD, Associate professor, Department of Rheumatology and Clinical Immunology, Graduate School of Medicine, Kyoto University.
³ From the Department of Rheumatology and Clinical Immunology, Graduate School of Medicine, Kyoto University, Kyoto; Department of Clinical Immunology and Rheumatology, Wakayama Medical University, Wakayama, Japan. ohmurako@kuhp.kyoto-u.ac.jp.
⁴ S. Nakabo, MD, research fellow, Department of Rheumatology and Clinical Immunology, Graduate School of Medicine, Kyoto University; H. Yoshifuji, MD, PhD, Assistant professor, Department of Rheumatology and Clinical Immunology, Graduate School of Medicine, Kyoto University; M. Hashimoto, MD, PhD, Assistant professor, Department of the Control for Rheumatic Diseases, Graduate School of Medicine, Kyoto University; Y. Imura, MD, PhD, Assistant professor, Department of Rheumatology and Clinical Immunology, Graduate School of Medicine, Kyoto University; R. Nakashima, MD, PhD, Assistant professor, Department of Rheumatology and Clinical Immunology, Graduate School of Medicine, Kyoto University; K. Murakami, MD, PhD, Assistant professor, Department of Rheumatology and Clinical Immunology, Graduate School of Medicine, Kyoto University; N. Kuramoto, MD, PhD, Assistant professor, Department of Rheumatology and Clinical Immunology, Graduate School of Medicine, Kyoto University; S. Ito, PhD, Assistant professor, Medical Research Support Center, Graduate School of Medicine, Kyoto University; J. Satoh, PhD, Postdoctoral fellow, Medical Research Support Center, Graduate School of Medicine, Kyoto University; M. Tanaka, MD, PhD, Associate professor, Department of the Control for Rheumatic Diseases, Graduate School of Medicine, Kyoto University; T. Fujii, MD, PhD, Professor, Department of Clinical Immunology and Rheumatology, Wakayama Medical University, and Department of Rheumatology and Clinical Immunology, Graduate School of Medicine, Kyoto University; T. Mimori, MD, PhD, Professor, Department of Rheumatology and Clinical Immunology, Graduate School of Medicine, Kyoto University; K. Ohmura, MD, PhD, Associate professor, Department of Rheumatology and Clinical Immunology, Graduate School of Medicine, Kyoto University. ohmurako@kuhp.kyoto-u.ac.jp.

PMID: 28507178
DOI: 10.3899/jrheum.161432

Abstract

Objective: Anti-carbamylated protein (anti-CarP) antibodies are possible diagnostic biomarkers of anticitrullinated protein antibody (ACPA)-negative rheumatoid arthritis (RA). We aimed to elucidate the prevalence of anti-CarP antibodies in non-RA connective tissue diseases (CTD) because CTD are important in the differential diagnosis of ACPA-negative RA.

Methods: The sera from 266 patients with RA and 616 patients with CTD and 80 healthy controls were examined using an in-house anti-CarP ELISA.

Results: The prevalence and the level of anti-CarP antibodies in several CTD were comparable to those in ACPA-negative RA.

Conclusion: Anti-CarP antibodies are not useful for differentiating ACPA-negative RA from CTD.

Keywords: ANTICYCLIC CITRULLINATED ANTIBODIES; AUTOANTIBODIES; CONNECTIVE TISSUE DISEASES; RHEUMATOID ARTHRITIS.

MeSH terms

Adult
Aged
Anti-Citrullinated Protein Antibodies / blood
Arthritis, Rheumatoid / blood
Arthritis, Rheumatoid / diagnosis*
Arthritis, Rheumatoid / immunology
Autoantibodies / blood*
Case-Control Studies
Connective Tissue Diseases / blood
Connective Tissue Diseases / diagnosis*
Connective Tissue Diseases / immunology
Diagnosis, Differential
Female
Humans
Male
Middle Aged
Rheumatoid Factor / blood

Substances

Anti-Citrullinated Protein Antibodies
Autoantibodies
Rheumatoid Factor