Application of Partially Substituted 3,5-Dimethylphenylcarbamate-(3-(2-O-β-Cyclodextrin)-2-Hydroxypropoxy)-Propylsilyl-Appended Silica Particles as Chiral Stationary Phase for Multi-mode High-performance Liquid Chromatography

Mingxuan Ma; Qunli Wei; Min Meng; Jiale Yin; Lei Du; Xia Zhu; Mengjun Min; Xueyan Zhou; Xiaoxing Yin; Yinhan Gong

doi:10.1093/chromsci/bmx044

Application of Partially Substituted 3,5-Dimethylphenylcarbamate-(3-(2-O-β-Cyclodextrin)-2-Hydroxypropoxy)-Propylsilyl-Appended Silica Particles as Chiral Stationary Phase for Multi-mode High-performance Liquid Chromatography

J Chromatogr Sci. 2017 Sep 1;55(8):839-845. doi: 10.1093/chromsci/bmx044.

Authors

Mingxuan Ma¹, Qunli Wei¹, Min Meng¹, Jiale Yin¹, Lei Du¹, Xia Zhu¹, Mengjun Min¹, Xueyan Zhou¹, Xiaoxing Yin¹, Yinhan Gong

Affiliation

¹ Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical University, 209 Tongshan Road, Xuzhou 221004, PR China.

PMID: 28505281
DOI: 10.1093/chromsci/bmx044

Abstract

A new type of partially substituted 3,5-dimethylphenylcarbamate-(3-(2-O-β-cyclodextrin)-2-hydroxypropoxy)-propylsilyl-appended silica particles (MP-CD-HPS) have been prepared by a convenient post-immobilization derivazition procedure. The MP-CD-HPS has been successfully used as chiral stationary phase (CSP) for high-performance liquid chromatography (HPLC) under normal phase, reversed phase and polar organic mobile phase conditions. The chromatographic evaluation results show that the MP-CD-HPS has excellent selectivity for the separation of aromatic positional isomers and enantiomers of some chiral compounds. The multi-mode HPLC separation results also indicate that both the stable ether spacer linking to the wider torus rim of β-cyclodextrin in the MP-CD-HPS phase and the hydroxyl residues in the partially substituted β-cyclodextrin have important contributions to chiral recognitions and chromatographic separations.