Bifidobacterial growth stimulation by oligosaccharides generated from olive tree pruning biomass

Encarnación Ruiz; Beatriz Gullón; Patrícia Moura; Florbela Carvalheiro; Gemma Eibes; Cristóbal Cara; Eulogio Castro

doi:10.1016/j.carbpol.2017.04.014

Bifidobacterial growth stimulation by oligosaccharides generated from olive tree pruning biomass

Carbohydr Polym. 2017 Aug 1:169:149-156. doi: 10.1016/j.carbpol.2017.04.014. Epub 2017 Apr 9.

Authors

Encarnación Ruiz¹, Beatriz Gullón², Patrícia Moura³, Florbela Carvalheiro⁴, Gemma Eibes⁵, Cristóbal Cara⁶, Eulogio Castro⁷

Affiliations

¹ Department of Chemical, Environmental and Materials Engineering, Universidad de Jaén, Campus Las Lagunillas, 23071, Jaén, Spain. Electronic address: eruiz@ujaen.es.
² Department of Chemical Engineering, Institute of Technology, University of Santiago de Compostela, 15782 Santiago de Compostela, Spain. Electronic address: beatriz.gullon@usc.es.
³ Unidade de Bioenergia, LNEG - Laboratório Nacional de Energia e Geologia, Estrada do Paço do Lumiar, 22, Lisboa 1649-038, Portugal. Electronic address: patricia.moura@lneg.pt.
⁴ Unidade de Bioenergia, LNEG - Laboratório Nacional de Energia e Geologia, Estrada do Paço do Lumiar, 22, Lisboa 1649-038, Portugal. Electronic address: florbela.carvalheiro@lneg.pt.
⁵ Department of Chemical Engineering, Institute of Technology, University of Santiago de Compostela, 15782 Santiago de Compostela, Spain. Electronic address: gemma.eibes@usc.es.
⁶ Department of Chemical, Environmental and Materials Engineering, Universidad de Jaén, Campus Las Lagunillas, 23071, Jaén, Spain. Electronic address: ccara@ujaen.es.
⁷ Department of Chemical, Environmental and Materials Engineering, Universidad de Jaén, Campus Las Lagunillas, 23071, Jaén, Spain. Electronic address: ecastro@ujaen.es.

PMID: 28504130
DOI: 10.1016/j.carbpol.2017.04.014

Abstract

This work aims to evaluate the prebiotic potential of oligosaccharides (OS) obtained from autohydrolysis of olive tree pruning biomass (OTPB). Two selected fractions (F1 and F2) were characterized and used in in vitro fermentations by two Bifidobacterium spp. (B. adolescentis and B. longum) and one fecal inoculum. The fraction F1 presented a lower average degree of polymerization (DP) mainly with OS ranging from 3 to 6 DP, whereas the fraction F2 corresponded to a pool of unsubstituted and acetylated oligomers with DP between 4 and 19. In the fermentation by Bifidobacterium, F1 supported a higher biomass formation, OS consumption and organic acids production than F2. With the fecal inoculum, the accumulation of organic acids, as the sum of acetate, propionate and butyrate, was similar for F1 and F2 (107 and 101mM, respectively). The bifidobacteria counts also increased during the incubation time for both OS fractions.

Keywords: Agricultural residues; FISH; Oligosaccharides; Prebiotics; SCFA.

MeSH terms

Bifidobacterium / drug effects
Bifidobacterium / growth & development*
Biomass
Feces
Fermentation
Olea / chemistry*
Oligosaccharides / chemistry*
Prebiotics*

Substances

Oligosaccharides
Prebiotics