One-pot chemo-enzymatic synthesis of furfuralcohol from xylose

Abstract

Furfuralcohol (FOL) is an important intermediate for the production of lysine, ascorbic acid, and lubricants. It can be used as a hypergolic fuel in rocketry. In this study, it was attempted to synthesize FOL from xylose by tandem catalysis with solid acid SO₄^2-/SnO₂-Montmorillonite and recombination Escherichia coli CCZU-K14 whole cells. Using SO₄^2-/SnO₂-Montmorillonite (3.0wt% dosage) as catalyst, a highest furfural yield of 41.9% was achieved from xylose at 170°C for 20min. Furthermore, Escherichia coli CCZU-K14 whole cells were used for bioconverting furfural to FOL. The optimum biocatalytic reaction temperature, reaction pH, cosubstrate concentration, and substrate concentration were 30°C, 6.5, 1.5mol glucose/mol furfural, and 200mM, respectively. Finally, the yield of FOL from 200mM furfural was achieved to 100% by Escherichia coli CCZU-K14 whole cells after 24h. In conclusion, this strategy show high potential application for the effective synthesis of FOL.

Keywords: Escherichia coli CCZU-K14; Furfural; Furfuralcohol; Reductase; SO(4)(2−)/SnO(2)-Montmorillonite.