Use of a Fluorescent Substrate to Measure ACE2 Activity in the Mouse Abdominal Aorta

Methods Mol Biol. 2017:1614:61-67. doi: 10.1007/978-1-4939-7030-8_5.

Abstract

The use of fluorogenic substrates to measure enzymatic activity is widely used to understand function within different experimental models. ACE2 is important in understanding the balance between AngII and Ang-(1-7) and how this balance could then in turn influence hypertension or other disease outcomes. Here, we describe a method to measure ACE2 activity in abdominal aorta of hyperlipidemic mice under both saline and AngII infusion.

Keywords: Activity; Angiotensin converting enzyme 2; Aorta; Enzyme; Fluorescence.

MeSH terms

  • Angiotensin II / chemistry
  • Angiotensin II / metabolism
  • Angiotensin-Converting Enzyme 2
  • Animals
  • Aorta, Abdominal / drug effects
  • Aorta, Abdominal / enzymology*
  • Disease Models, Animal
  • Fluorescent Antibody Technique / methods*
  • Fluorescent Dyes / metabolism*
  • Hyperlipidemias / enzymology
  • Hyperlipidemias / pathology
  • Mice
  • Mice, Knockout
  • Peptidyl-Dipeptidase A / analysis*
  • Peptidyl-Dipeptidase A / metabolism
  • Receptors, LDL / physiology
  • Sodium Chloride / administration & dosage
  • Vasoconstrictor Agents / metabolism

Substances

  • Fluorescent Dyes
  • Receptors, LDL
  • Vasoconstrictor Agents
  • Angiotensin II
  • Sodium Chloride
  • Peptidyl-Dipeptidase A
  • Ace2 protein, mouse
  • Angiotensin-Converting Enzyme 2