Erythrocyte membrane nanoparticles improve the intestinal absorption of paclitaxel

Xing Jiang; Kaikai Wang; Zaigang Zhou; Yifan Zhang; Huizi Sha; Qiuping Xu; Jie Wu; Juan Wang; Jinhui Wu; Yiqiao Hu; Baorui Liu

doi:10.1016/j.bbrc.2017.05.042

Erythrocyte membrane nanoparticles improve the intestinal absorption of paclitaxel

Biochem Biophys Res Commun. 2017 Jun 24;488(2):322-328. doi: 10.1016/j.bbrc.2017.05.042. Epub 2017 May 8.

Authors

Xing Jiang¹, Kaikai Wang², Zaigang Zhou³, Yifan Zhang³, Huizi Sha⁴, Qiuping Xu⁴, Jie Wu³, Juan Wang³, Jinhui Wu⁵, Yiqiao Hu⁶, Baorui Liu⁷

Affiliations

¹ The Comprehensive Cancer Center of Drum Tower Hospital, Clinical College of Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing 210008, China; College of Nursing, Nanjing University of Chinese Medicine, Nanjing 210023, China; The Comprehensive Cancer Center of Drum Tower Hospital, Medical School of Nanjing University & Clinical Cancer Institute of Nanjing University, Nanjing 210008, China.
² State Key Laboratory of Pharmaceutical Biotechnology, Medical School, Nanjing University, Nanjing 210093, China; Department of Pharmaceutics, China Pharmaceutical University, Nanjing 210028, China.
³ State Key Laboratory of Pharmaceutical Biotechnology, Medical School, Nanjing University, Nanjing 210093, China.
⁴ The Comprehensive Cancer Center of Drum Tower Hospital, Medical School of Nanjing University & Clinical Cancer Institute of Nanjing University, Nanjing 210008, China.
⁵ State Key Laboratory of Pharmaceutical Biotechnology, Medical School, Nanjing University, Nanjing 210093, China. Electronic address: wuj@nju.edu.cn.
⁶ State Key Laboratory of Pharmaceutical Biotechnology, Medical School, Nanjing University, Nanjing 210093, China. Electronic address: huyiqiao@nju.edu.cn.
⁷ The Comprehensive Cancer Center of Drum Tower Hospital, Clinical College of Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing 210008, China; The Comprehensive Cancer Center of Drum Tower Hospital, Medical School of Nanjing University & Clinical Cancer Institute of Nanjing University, Nanjing 210008, China. Electronic address: baoruiliu@nju.edu.cn.

PMID: 28495530
DOI: 10.1016/j.bbrc.2017.05.042

Abstract

Paclitaxel (PTX) is a cytotoxic chemotherapy drug with encouraging activity in human malignancies. However, free PTX has a very low oral bioavailability due to its low aqueous solubility and the gastrointestinal drug barrier. In order to overcome this obstacle, we have designed erythrocyte membrane nanoparticles (EMNP) using sonication method. The permeability of PTX by EMNP was 3.5-fold (P_app = 0.425 nm/s) and 16.2-fold (P_app = 394.1 nm/s) higher than free PTX in MDCK-MDR1 cell monolayers and intestinal mucosal tissue, respectively. The in vivo pharmacokinetics indicated that the AUC_0-t (μg/mL·h) and C_max (μg/mL) of EMNP were 14.2-fold and 6.0-fold higher than that of free PTX, respectively. In summary, the EMNP appears to be a promising nanoformulation to enhance the oral bioavailability of insoluble and poorly permeable drugs.

Keywords: Drug delivery; Erythrocyte membrane; Oral route; Paclitaxel.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antineoplastic Agents, Phytogenic / administration & dosage*
Antineoplastic Agents, Phytogenic / chemistry
Antineoplastic Agents, Phytogenic / pharmacology*
Cell Survival / drug effects
Dose-Response Relationship, Drug
Erythrocyte Membrane / chemistry*
Erythrocyte Membrane / metabolism
Intestinal Absorption / drug effects*
Nanoparticles / chemistry*
Nanoparticles / metabolism
Paclitaxel / administration & dosage*
Paclitaxel / chemistry
Paclitaxel / pharmacology*
Particle Size
Rats
Rats, Sprague-Dawley
Structure-Activity Relationship
Surface Properties

Substances

Antineoplastic Agents, Phytogenic
Paclitaxel