The Determination of Sugars in Dairy Products: Development of a New Standard Method for the International Dairy Federation and the Internal Organization for Standardization

J AOAC Int. 2017 Sep 1;100(5):1577-1581. doi: 10.5740/jaoacint.17-0068. Epub 2017 May 11.

Abstract

A method using high-performance anion-exchange chromatography (HPAEC) with a pulsed amperometric detector (PAD) for the determination of mono- and disaccharides is described. The method was accepted by the International Dairy Federation and the Internal Organization for Standardization as a new work item for the determination of sugars in dairy matrixes, and the Milk and Milk Products technical committee of ISO/TC 34/SC 5 accepted the topic "Milk and milk products - Determination of the sugar contents - High-performance anion-exchange chromatographic method (HPAEC-PAD)" as a new work item. The proposed method consists of an aqueous ethanol extraction of the sugars in the dairy sample, followed by clarification with Carrez I and II reagents. The clarified filtrate is diluted and then directly introduced in the HPAEC-PAD system for quantification of the sugars. A single-laboratory validation of the proposed method has been scheduled for spring 2017.

MeSH terms

  • Animals
  • Carbohydrates / analysis*
  • Chromatography, High Pressure Liquid / standards*
  • Chromatography, Ion Exchange / standards*
  • Dairy Products / analysis*
  • Milk / chemistry*

Substances

  • Carbohydrates