Residue analysis of tetracyclines in milk by HPLC coupled with hollow fiber membranes-based dynamic liquid-liquid micro-extraction

Hui Xu; Hong-Yu Mi; Ming-Ming Guan; Hong-Yan Shan; Qiang Fei; Yan-Fu Huan; Zhi-Quan Zhang; Guo-Dong Feng

doi:10.1016/j.foodchem.2017.04.021

Residue analysis of tetracyclines in milk by HPLC coupled with hollow fiber membranes-based dynamic liquid-liquid micro-extraction

Food Chem. 2017 Oct 1:232:198-202. doi: 10.1016/j.foodchem.2017.04.021. Epub 2017 Apr 5.

Authors

Hui Xu¹, Hong-Yu Mi², Ming-Ming Guan³, Hong-Yan Shan⁴, Qiang Fei⁵, Yan-Fu Huan⁶, Zhi-Quan Zhang⁷, Guo-Dong Feng⁸

Affiliations

¹ Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun 130021, PR China. Electronic address: xuhui1418@163.com.
² Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun 130021, PR China. Electronic address: mihongyu1992@163.com.
³ Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun 130021, PR China. Electronic address: 15530839940@163.com.
⁴ Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun 130021, PR China. Electronic address: shanhy@jlu.edu.cn.
⁵ Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun 130021, PR China. Electronic address: feiqiang@jlu.edu.cn.
⁶ Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun 130021, PR China. Electronic address: yfhuan@jlu.edu.cn.
⁷ Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun 130021, PR China. Electronic address: zzq@jlu.edu.cn.
⁸ Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun 130021, PR China. Electronic address: fenggd@jlu.edu.cn.

PMID: 28490065
DOI: 10.1016/j.foodchem.2017.04.021

Abstract

A novel hollow fiber membranes-based dynamic liquid-liquid micro-extraction (HF-DLLME) coupled with HPLC-UV detection has been developed for the residue analysis of tetracyclines in milk samples without deproteinization and degreasing. The influences of experimental parameters were investigated and optimized. The method showed a good performance. The limits of detection (LOD) are in the range of 0.95-3.6μg/L. The recoveries in spiked samples range from 92.38 to 107.3%. The relative standard deviations (RSDs) are lower than 8.66%. The advantages of this method are simple operation, high efficiency, absence of sample carryover and low cost.

Keywords: Chlorotetracycline (PubChem CID: 54675777); Doxycycline (PubChem CID: 54671203); Dynamic liquid-liquid micro-extraction; Hollow fiber membrane; Ionic liquid; Milk; Oxytetracycline (PubChem CID: 54675779); Tetracycline (PubChem CID: 54675776); Tetracyclines.

MeSH terms

Animals
Chromatography, High Pressure Liquid
Food Analysis
Food Contamination
Liquid Phase Microextraction
Liquid-Liquid Extraction
Milk*
Tetracyclines*

Substances

Tetracyclines