Iodine-loaded metal organic framework as growth-triggered antimicrobial agent

Abstract

Zeolitic imidazolate framework-8 (ZIF-8) is one of easily available metal organic frameworks because of its facile preparation via mixing aqueous solutions of zinc nitrate and 2-methylimidazole. It turned into a very effective pH-responsive bactericide after loading with iodine. Approximately, 0.9g of iodine could be readily loaded into one gram of ZIF-8 from iodine dissolved n-heptane solution. Both Gram-negative Escherichia coli and Gram-positive Staphylococcus epidermidis and Staphylococcus aureus could be very effectively killed by iodine loaded ZIF-8 (ZIF-8@I) at pH6.0 within 3min. In contrast, at pH above 7.0, no appreciable antimicrobial activity could be detected. The bacteria killing effect is resulted from the iodine released from ZIF-8@I disintegrated at acidic pH. ZIF-8@I coated surface also showed its acidic pH-triggered antimicrobial activity against deposited bacterial cells. The antimicrobial activity of ZIF-8@I against actively grown bacterial lawns on a pH neutral agar plate was also observed. The result demonstrates that iodine was released from the disintegrated ZIF-8@I to kill bacteria in response to the bacterial growth-induced pH lowering.

Keywords: Antibacterial agent; Iodine adsorption; Zeolitic imidazolate framework-8 (ZIF-8); pH-responsive bactericide.