Spontaneous and efficient adsorption of lysozyme from aqueous solutions by naturally polyanion gel beads

Jiwei Li; Yang Si; Cunyi Zhao; Jinmei He; Gang Sun; Yudong Huang

doi:10.1016/j.msec.2017.03.092

Spontaneous and efficient adsorption of lysozyme from aqueous solutions by naturally polyanion gel beads

Mater Sci Eng C Mater Biol Appl. 2017 Jul 1:76:130-138. doi: 10.1016/j.msec.2017.03.092. Epub 2017 Mar 14.

Authors

Jiwei Li¹, Yang Si², Cunyi Zhao², Jinmei He³, Gang Sun⁴, Yudong Huang⁵

Affiliations

¹ MIIT Key Laboratory of Critical Materials Technology for New Energy Conversion and Storage, School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin 150001, China; Fiber and Polymer Science, University of California, Davis, CA 95616, USA.
² Fiber and Polymer Science, University of California, Davis, CA 95616, USA.
³ MIIT Key Laboratory of Critical Materials Technology for New Energy Conversion and Storage, School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin 150001, China. Electronic address: hejinmei@hit.edu.cn.
⁴ Fiber and Polymer Science, University of California, Davis, CA 95616, USA. Electronic address: gysun@ucdavis.edu.
⁵ MIIT Key Laboratory of Critical Materials Technology for New Energy Conversion and Storage, School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin 150001, China.

PMID: 28482498
DOI: 10.1016/j.msec.2017.03.092

Abstract

In this study, polyanionic alginate gel beads crosslinked by Ca²⁺ and glutaraldehyde have demonstrated a strong electrostatic interaction with specific proteins. Due to the naturally abundant carboxyl groups, the prepared alginate gel beads exhibited a relatively superior integrated adsorption performance toward lysozyme, including a superior adsorption capacity of 213mgg^-1, fast adsorption equilibrium within 12h, good selectivity, and good reversibility. Compared with other protein adsorbents, the as-prepared adsorptive beads have the advantages of excellent adsorption performance, easy to prepare, convenient, efficient, reliable and environment-friendly to apply, which can serve as a more sustainable material in protein separation and purification.

Keywords: Adsorption; Alginate; Calcium; Electrostatic interaction; Lysozyme.

MeSH terms

Adsorption
Alginates
Muramidase / metabolism*
Polyelectrolytes
Polymers

Substances

Alginates
Polyelectrolytes
Polymers
polyanions
Muramidase