Bloodstream bacterial infections are known to illicit a systemic immune response that can lead to multiorgan failure and septic shock. The current endotoxin identification techniques in serum are expensive and elaborate requiring bulky benchtop instrumentation. We demonstrate a new route for endotoxin detection in which lipopolysaccharides (LPS) in solution are entrapped using C-18 silane-functionalized glass slides and tagged with polymyxin B sulfate (PMB) drug-conjugated gold nanoparticles. The signal from the particles is further amplified via the silver reduction approach to yield concentration-dependent colorimetric spots visible to the bare eye. The method is rapid, reliable, and cost-effective and fulfills an urgent unmet need in the healthcare industry for early septicemia diagnosis.
Keywords: C-18 silane; Endotoxin; Gold nanoparticle (GNP); Lipopolysaccharide (LPS); Polymyxin B sulfate (PMB); Silver enhancement.