Fourier Transform Infrared Spectroscopy as a Tool in Analysis of Proteus mirabilis Endotoxins

Paulina Żarnowiec; Grzegorz Czerwonka; Wiesław Kaca

doi:10.1007/978-1-4939-6958-6_11

Fourier Transform Infrared Spectroscopy as a Tool in Analysis of Proteus mirabilis Endotoxins

Methods Mol Biol. 2017:1600:113-124. doi: 10.1007/978-1-4939-6958-6_11.

Authors

Paulina Żarnowiec¹, Grzegorz Czerwonka¹, Wiesław Kaca²

Affiliations

¹ Department of Microbiology, Jan Kochanowski University, Świętokrzyska 15, PL-25-406, Kielce, Poland.
² Department of Microbiology, Jan Kochanowski University, Świętokrzyska 15, PL-25-406, Kielce, Poland. wieslaw.kaca@ujk.edu.pl.

PMID: 28478562
DOI: 10.1007/978-1-4939-6958-6_11

Abstract

Fourier transform infrared spectroscopy (FT-IR) was used to scan whole bacterial cells as well as lipopolysaccharides (LPSs, endotoxins) isolated from them. Proteus mirabilis cells, with chemically defined LPSs, served as a model for the ATR FT-IR method. The paper focuses on three steps of infrared spectroscopy: (1) sample preparation, (2) IR scanning, and (3) multivariate analysis of IR data (principal component analysis, PCA).

Keywords: ATR FT-IR spectroscopy; Fourier transform infrared spectroscopy; Lipopolysaccharides; Multivariate analysis; Principal component analysis.

MeSH terms

Endotoxins / analysis*
Lipopolysaccharides / analysis
Multivariate Analysis
Principal Component Analysis
Proteus mirabilis / metabolism*
Spectroscopy, Fourier Transform Infrared / methods*

Substances

Endotoxins
Lipopolysaccharides