Electrochemical Aptamer Scaffold Biosensors for Detection of Botulism and Ricin Proteins

Jessica Daniel; Lisa Fetter; Susan Jett; Teisha J Rowland; Andrew J Bonham

doi:10.1007/978-1-4939-6958-6_2

Electrochemical Aptamer Scaffold Biosensors for Detection of Botulism and Ricin Proteins

Methods Mol Biol. 2017:1600:9-23. doi: 10.1007/978-1-4939-6958-6_2.

Authors

Jessica Daniel¹, Lisa Fetter¹, Susan Jett¹, Teisha J Rowland², Andrew J Bonham³

Affiliations

¹ Department of Chemistry, Metropolitan State University of Denver, 890 Auraria Parkway, Denver, CO, 80220, USA.
² Cardiovascular Institute and Adult Medical Genetics Program, University of Colorado Denver Anschutz Medical Campus, 12700 E 19th Ave, Aurora, 80045, CO, USA.
³ Department of Chemistry, Metropolitan State University of Denver, 890 Auraria Parkway, Denver, CO, 80220, USA. abonham@msudenver.edu.

PMID: 28478553
DOI: 10.1007/978-1-4939-6958-6_2

Abstract

Electrochemical DNA (E-DNA) biosensors enable the detection and quantification of a variety of molecular targets, including oligonucleotides, small molecules, heavy metals, antibodies, and proteins. Here we describe the design, electrode preparation and sensor attachment, and voltammetry conditions needed to generate and perform measurements using E-DNA biosensors against two protein targets, the biological toxins ricin and botulinum neurotoxin. This method can be applied to generate E-DNA biosensors for the detection of many other protein targets, with potential advantages over other systems including sensitive detection limits typically in the nanomolar range, real-time monitoring, and reusable biosensors.

Keywords: Aptamer; Biosensors; Botulism; E-DNA; Electrochemical; Gold electrodes; Proteins; Ricin; Toxins; Voltammetry.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aptamers, Nucleotide / chemistry*
Biosensing Techniques / methods*
Botulism / diagnosis*
Botulism / metabolism*
Electrochemical Techniques / methods*
Ricin / analysis*

Substances

Aptamers, Nucleotide
Ricin