Information on the spatio-temporal variability of soil moisture in the vadose zone is important to assess groundwater recharge and solute transport in unconsolidated substrate as influenced by biological processes. Time-lapse electrical resistivity imaging (ERI) was used to monitor soil moisture dynamics to a depth of 9 m in a grassland, a grassland encroached by a juniper species (eastern redcedar, Juniperus virginiana), a juniper woodland and an oak forest in the south-central Great Plains, Oklahoma, USA. A site-specific relationship between moisture content and electrical conductivity data was developed for the soil zone, and a perched water zone was monitored at two of the sites. Results showed that (a) change in soil moisture content was linearly correlated to change in electric conductivity in the soil zone; (b) vegetation cover type induced differences in vertical bulk electrical resistivity (ER) profiles and influenced the temporal evolution of soil moisture profiles; and (c) juniper encroachment lowered the water level in the perched groundwater aquifer. Our results suggest land use and vegetation cover type, as opposed to rock properties, controls deep water drainage for the vegetation transition zone. Methods used to measure hydrogeophysical changes, such as ERI, can be used for broader understanding of geological, physical, and biological processes and their links in Earth's critical zones.