Detection of cytomegalovirus in shell vial cultures by using a DNA probe and early nuclear antigen monoclonal antibody

A A Scott; K A Walker; L M Hennigar; C H Williams; J P Manos; T Gansler

doi:10.1128/jcm.26.9.1895-1897.1988

Detection of cytomegalovirus in shell vial cultures by using a DNA probe and early nuclear antigen monoclonal antibody

J Clin Microbiol. 1988 Sep;26(9):1895-7. doi: 10.1128/jcm.26.9.1895-1897.1988.

Authors

A A Scott¹, K A Walker, L M Hennigar, C H Williams, J P Manos, T Gansler

Affiliation

¹ Department of Pathology and Laboratory Medicine, Medical University of South Carolina, Charleston 29425.

Abstract

An in situ biotinylated DNA probe assay was evaluated as an adjunct to anti-cytomegalovirus early nuclear antigen indirect immunofluorescence and cytopathic effect on cytomegalovirus-infected monolayers in shell vial cultures. Viral infection was detected by early nuclear antigen indirect immunofluorescence at 24 h and by DNA probe assay and shell vial cytopathic effect at 5 days.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Antibodies, Monoclonal*
Antigens, Viral / analysis*
Cell Line
Cytomegalovirus / genetics
Cytomegalovirus / immunology
Cytomegalovirus / isolation & purification*
Cytopathogenic Effect, Viral
DNA Probes*
DNA, Viral / analysis*
Fibroblasts
Fluorescent Antibody Technique
Immediate-Early Proteins*
Nucleic Acid Hybridization
Predictive Value of Tests

Substances

Antibodies, Monoclonal
Antigens, Viral
DNA Probes
DNA, Viral
Immediate-Early Proteins
immediate-early proteins, cytomegalovirus