Anti-proliferative ambuic acid derivatives from Hawaiian endophytic fungus Pestalotiopsis sp. FT172

Phytochemistry. 2017 Aug:140:77-82. doi: 10.1016/j.phytochem.2017.04.017.

Abstract

Five previously undescribed ambuic acid derivatives, pestallic acids A-E and three known analogs were isolated from the cultured broth of Pestalotiopsis sp. FT172. The structures of the pestallic acids A-E were determined through the analysis of HRMS and NMR spectroscopic data. The absolute configurations (ACs) of pestallic acids B-E were assigned by comparison of the experimental electric circular dichroism (ECD) spectra or the optical rotations with those in the literature. All compounds were tested against A2780 and cisplatin resistant A2780 (A2780CisR) cell lines. Pestallic acid E and (+)-ambuic acid showed potent activities with IC50 values from 3.3 to 17.0 μM.

Keywords: Ambuic acid; Antiproliferative; Endophytic fungi; Myrsinaceae; Myrsine sandwicensis; Pestalotiopsis.

MeSH terms

  • Antineoplastic Agents, Phytogenic / chemistry*
  • Antineoplastic Agents, Phytogenic / isolation & purification
  • Cell Line, Tumor
  • Cyclohexanones / chemistry*
  • Cyclohexanones / isolation & purification
  • Endophytes / chemistry
  • Hawaii
  • Humans
  • Molecular Structure
  • Primulaceae / microbiology
  • Xylariales / chemistry*

Substances

  • Antineoplastic Agents, Phytogenic
  • Cyclohexanones
  • ambuic acid