Data on the uptake and metabolism of testosterone by the common mussel, Mytilus spp

Tamar I Schwarz; Ioanna Katsiadaki; Benjamin H Maskrey; Alexander P Scott

doi:10.1016/j.dib.2017.03.040

Data on the uptake and metabolism of testosterone by the common mussel, Mytilus spp

Data Brief. 2017 Mar 31:12:164-168. doi: 10.1016/j.dib.2017.03.040. eCollection 2017 Jun.

Authors

Tamar I Schwarz¹, Ioanna Katsiadaki¹, Benjamin H Maskrey¹, Alexander P Scott¹

Affiliation

¹ Centre for Environment, Fisheries and Aquaculture Science, Barrack Road, Weymouth, Dorset DT4 8UB, UK.

Abstract

This article provides data in support of the research article entitled "Rapid uptake, biotransformation, esterification and lack of depuration of testosterone and its metabolites by the common mussel, Mytilus spp." (T.I. Schwarz, I. Katsiadaki, B.H. Maskrey, A.P. Scott, 2017) [1]. The uptake of tritiated testosterone (T) from water by mussels is presented. The two main radioactive peaks formed from T and present in the fatty acid ester fraction of mussel tissues were shown to have the same elution positions on a thin layer chromatography plate as 17β-hydroxy-5α-androstan-3-one (DHT) and 5α-androstan-3β,17β-diol (3β,17β-A5α). Reverse phase high performance liquid chromatography of the non-esterified (80% ethanol) fraction of the mussel tissue extracts also presented radioactive peaks at the elution positions of DHT and 3β,17β-A5α. There was no evidence for sulfated T in this fraction. It was shown that aeration led to significant losses of radiolabeled testosterone from the water column.

Keywords: Mollusc; Steroid metabolism, androgen; Sulphate.