Potential Involvement of SCF-Complex in Zygotic Genome Activation During Early Bovine Embryo Development

Methods Mol Biol. 2017:1605:245-257. doi: 10.1007/978-1-4939-6988-3_17.

Abstract

Proper timing of degradation of maternal protein reserves is important for early embryonic development. The major modification that triggers proteins to degradation is ubiquitination, mediated by ubiquitin-proteolytic system. We focus here on Skp 1-Cul 1-F-box complex (SCF-complex), E3 ubiquitin-ligase, a part of ubiquitin-proteolytic system, which transfer ubiquitin to the substrate protein. We describe in this chapter the methods for the characterization of the expression profile of mRNA and protein of invariant members of SCF-complex and for the definition of SCF-complex activity.

Keywords: Immunofluorescence; In situ proximity ligation assay; Preimplantation development; SCF-complex; Western blot; qRT-PCR.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cattle
  • Embryonic Development*
  • Female
  • Gene Expression Profiling
  • Gene Expression Regulation, Developmental
  • Proteolysis
  • SKP Cullin F-Box Protein Ligases / metabolism*
  • Transcriptional Activation
  • Ubiquitin-Protein Ligases / metabolism*

Substances

  • SKP Cullin F-Box Protein Ligases
  • Ubiquitin-Protein Ligases