Th17 cell-mediated immune responses promote mast cell proliferation by triggering stem cell factor in keratinocytes

Biochem Biophys Res Commun. 2017 Jun 10;487(4):856-861. doi: 10.1016/j.bbrc.2017.04.141. Epub 2017 Apr 27.

Abstract

Although mast cells are traditionally thought to function as effector cells in allergic responses, they have increasingly been recognized as important regulators of various immune responses. Mast cells mature locally; thus, tissue-specific influences are important for promoting mast cell accumulation and survival in the skin and the gastrointestinal tract. In this study, we determined the effects of keratinocytes on mast cell accumulation during Th17-mediated skin inflammation. We observed increases in dermal mast cells in imiquimod-induced psoriatic dermatitis in mice accompanied by the expression of epidermal stem cell factor (SCF), a critical mast cell growth factor. Similar to mouse epidermal keratinocytes, SCF was highly expressed in the human HaCaT keratinocyte cell line following stimulation with IL-17. Further, keratinocytes promoted mast cell proliferation following stimulation with IL-17 in vitro. However, the effects of keratinocytes on mast cells were significantly diminished in the presence of anti-CD117 (stem cell factor receptor) blocking antibodies. Taken together, our results revealed that the Th17-mediated inflammatory environment promotes mast cell accumulation through keratinocyte-derived SCF.

Keywords: Keratinocyte; Mast cell; Psoriasis; Stem cell factor.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line
  • Cell Proliferation
  • Humans
  • Keratinocytes / metabolism*
  • Mast Cells / cytology*
  • Mast Cells / immunology*
  • Stem Cell Factor / metabolism*
  • Th17 Cells / immunology*

Substances

  • Stem Cell Factor