An unusual spinaceamine-bearing pregnane from a soft coral Scleronephthya sp. inhibits the migration of tumor cells

Bioorg Med Chem Lett. 2017 Jun 15;27(12):2736-2741. doi: 10.1016/j.bmcl.2017.04.058. Epub 2017 Apr 20.

Abstract

An unprecedented spinaceamine-bearing pregnane namely scleronine (1) was isolated from a Chinese soft coral Scleronephthya sp. Its structure was determined on the basis of 1D and 2D NMR spectroscopic analyses in association with the HRESIMS data, while the absolute configurations were deduced by the single-crystal X-ray diffraction analysis. In addition, a dehydrogenated analogue (3) was synthesized through six steps with pregna-1,20-dien-3-one (2) as a precursor. The significantly inhibitory effects of 1 and 3 against the migration of tumor cells A549 and B16 accompanying the down-regulation of key genes (TGFβ, TNFα, IL-1β, and IL-6) were observed. These findings suggested that both 1 and 3 are potential for therapeutic usage aiming at cancer metastasis inhibition.

Keywords: Metastasis inhibition; Scleronephthya sp.; Scleronine; Soft coral; Synthesis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anthozoa / chemistry*
  • Cell Line, Tumor
  • Cell Movement / drug effects
  • Dose-Response Relationship, Drug
  • Heterocyclic Compounds, 2-Ring / chemistry
  • Heterocyclic Compounds, 2-Ring / isolation & purification
  • Heterocyclic Compounds, 2-Ring / pharmacology*
  • Humans
  • Molecular Structure
  • Pregnanes / chemistry
  • Pregnanes / isolation & purification
  • Pregnanes / pharmacology*
  • Quantum Theory
  • Structure-Activity Relationship

Substances

  • Heterocyclic Compounds, 2-Ring
  • Pregnanes
  • scleronine