Label-free quantitative proteomic analysis of inhibition of Xanthomonas axonopodis pv. citri by the novel bactericide Fubianezuofeng

Pestic Biochem Physiol. 2017 May:138:37-42. doi: 10.1016/j.pestbp.2017.02.004. Epub 2017 Feb 24.

Abstract

To understand the antibacterial mechanism of the new bactericide 2-(methylsulfonyl)-5- (4-fluorobenzyl)-1, 3, 4-oxadiazole (Generic name: Fubianezuofeng), we performed label-free quantitative proteomics analysis of the response of Xanthomonas axonopodis pv. citri (Xac) strain 29-1 to Fubianezuofeng. A total of 1133 proteins were identified in the treatment and control groups. Upon treatment with the 1/2 minimum inhibitory concentration (MIC), 339 proteins were found to be differentially expressed (fold changes>1.5, p<0.05) with 99 upregulated and 240 down-regulated. In comparison, 314 proteins were differentially expressed (125 up-regulated, 189 down-regulated) at MIC. The differentially expressed proteins were enriched for those involved in the pyrimidine metabolic pathway. The results offer a complete view of the proteome changes in bacteria in response to Fubianezuofeng.

Keywords: Differentially expressed protein; Fubianezuofeng; Quantitative proteomics; Xanthomonas axonopodis pv. citri.

MeSH terms

  • Anti-Bacterial Agents / chemistry
  • Anti-Bacterial Agents / pharmacology*
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism
  • Gene Expression Regulation, Bacterial / drug effects
  • Molecular Structure
  • Oxadiazoles / chemistry
  • Oxadiazoles / pharmacology*
  • Protein Interaction Maps
  • Proteomics*
  • Sulfones / chemistry
  • Sulfones / pharmacology*
  • Xanthomonas axonopodis / classification*
  • Xanthomonas axonopodis / drug effects*

Substances

  • Anti-Bacterial Agents
  • Bacterial Proteins
  • Oxadiazoles
  • Sulfones
  • fubianezuofeng