Long-term impacts of titanium dioxide nanoparticles (TiO2 NPs) on performance and microbial community of activated sludge

Abstract

The long-term impacts of titanium dioxide nanoparticles (TiO₂ NPs) on the performance and microbial community of activated sludge were evaluated in a sequencing batch reactor (SBR). TiO₂ NPs impacted the COD and phosphorus removals of activated sludge, whereas the NH₄⁺-N removal efficiency had no distinct change at 0-60mg/L TiO₂ NPs. The presence of TiO₂ NPs obviously inhibited the organic matter and nitrogen removal rates of activated sludge. The phosphorus removal rate gradually reduced at 0-5mg/L TiO₂ NPs and then increased at 10-60mg/L TiO₂ NPs. The removal rates of organic matter, nitrogen and phosphorus had the similar varying trends to the corresponding microbial enzymatic activities. High TiO₂ NPs concentration promoted more reactive oxygen species (ROS) production and lactate dehydrogenase (LDH) release of activated sludge. The microbial richness and diversity of activated sludge were obviously affected at the phyla, class and genus levels.

Keywords: Activated sludge; LDH; Microbial community; ROS; TiO(2) NPs.