Optimisation of Pueraria isoflavonoids by response surface methodology using ultrasonic-assisted extraction

Ka H Wong; George Q Li; Kong M Li; Valentina Razmovski-Naumovski; Kelvin Chan

doi:10.1016/j.foodchem.2017.03.068

Optimisation of Pueraria isoflavonoids by response surface methodology using ultrasonic-assisted extraction

Food Chem. 2017 Sep 15:231:231-237. doi: 10.1016/j.foodchem.2017.03.068. Epub 2017 Mar 14.

Authors

Ka H Wong¹, George Q Li², Kong M Li³, Valentina Razmovski-Naumovski⁴, Kelvin Chan⁵

Affiliations

¹ Faculty of Pharmacy, The University of Sydney, NSW 2006, Australia. Electronic address: kwon7030@uni.sydney.edu.au.
² Faculty of Pharmacy, The University of Sydney, NSW 2006, Australia. Electronic address: george.li@sydney.edu.au.
³ Discipline of Pharmacology, Bosch Institute, The University of Sydney, NSW 2006, Australia. Electronic address: kong.m.li@sydney.edu.au.
⁴ Faculty of Pharmacy, The University of Sydney, NSW 2006, Australia; The National Institute of Complementary Medicine (NICM), Western Sydney University, NSW, Australia; South Western Sydney Clinical School, School of Medicine, University of New South Wales, Australia. Electronic address: V.Naumovski@westernsydney.edu.au.
⁵ Faculty of Pharmacy, The University of Sydney, NSW 2006, Australia; The National Institute of Complementary Medicine (NICM), Western Sydney University, NSW, Australia; School of Pharmacy & Biomolecular Sciences, Liverpool John Moores University, Liverpool L3 3AF, United Kingdom. Electronic address: k.k.chan@ljmu.ac.uk.

PMID: 28450001
DOI: 10.1016/j.foodchem.2017.03.068

Abstract

Puerariae Lobatae Radix (PLR) exerts cyto-protective effect against oxidative stress due to its high isoflavonoid content. In this study, the ultrasonic-assisted extraction condition for the maximum recovery of isoflavonoids with high cyto-protective effect was optimised by response surface methodology (RSM). A second-order polynomial fitted the experimental data (R²: 0.9736; p-value <0.0001). The optimal extraction parameters were determined as: extraction time 16.02min, ethanol concentration 41.41% and liquid-to-solid ratio 44.35mL/g. Practical experiments with extraction time 16.00min, ethanol concentration 41.00% and liquid-to-solid ratio 44.00mL/g were carried out in triplicate. This subsequently yielded a cell viability of 82.90±0.78% against hydrogen peroxide-induced oxidative stress on EA.hy926, and was comparable to the predicted of 85.60%. Five chemical constituents in the extract were identified to exert cyto-protective effect. Taken together, this method successfully integrated RSM and the partial least squares regression method to optimise the PLR extract with highest cyto-protective activity.

Keywords: Pueraria lobata; Puerariae Lobatae Radix; Response surface methodology; Ultrasonic-assisted extraction.

MeSH terms

Chromatography, High Pressure Liquid
Flavonoids*
Least-Squares Analysis
Plant Roots
Pueraria*
Ultrasonics*

Substances

Flavonoids