1,6-O,O-Diacetylbritannilactone suppresses activation of mast cell and airway hyper-responsiveness

Meihua Jin; Sungun Kim; Nan Qin; Xi Chen; Ning Ji; Sheng-An Tang; Dexin Kong; Eunkyung Lee; Hongquan Duan

doi:10.1080/08923973.2017.1318911

1,6-O,O-Diacetylbritannilactone suppresses activation of mast cell and airway hyper-responsiveness

Immunopharmacol Immunotoxicol. 2017 Aug;39(4):173-179. doi: 10.1080/08923973.2017.1318911. Epub 2017 Apr 27.

Authors

Meihua Jin¹, Sungun Kim², Nan Qin³, Xi Chen^{1

4}, Ning Ji¹, Sheng-An Tang¹, Dexin Kong^{1

3}, Eunkyung Lee², Hongquan Duan^{1

3}

Affiliations

¹ a Tianjin Key Laboratory on Technologies Enabling, Development of Clinical Therapeutics and Diagnostics, School of Pharmacy , Tianjin Medical University , Tianjin , People's Republic of China.
² b Traditional Korean Medicine Technology Division, National Development Institute of Korean Medicine , Gyeongsan , Republic of Korea.
³ c Research Center of Basic Medical Sciences, Tianjin Medical University , Tianjin , People's Republic of China.
⁴ d Tianjin Key Laboratory of Ophthalmology and Visual Science , Tianjin Eye Hospital , Tianjin , People's Republic of China.

PMID: 28447503
DOI: 10.1080/08923973.2017.1318911

Abstract

Mast cells play critical roles in allergic disorders such as atopic dermatitis and allergic asthma. The aim of this study was to investigate the anti-inflammatory and anti-asthmatic activities of 1,6-O,O-diacetylbritannilactone (OODBL) isolated from Inula japonica Thunb. (I. japonica) in a murine asthma model and bone marrow-derived mast cells (BMMCs). In an ovalbumin-induced asthma model, OODBL administration attenuated the airway hyper-responsiveness induced by aerosolized methacholine and serum IgE level in asthmatic mice. In vitro system, we found that OODBL reduced leukotriene C₄ production and degranulation through the suppression of cytosolic phospholipase A₂ phosphorylation and phospholipase Cγ-mediated Ca²⁺ influx in IgE/antigen-stimulated BMMCs. Taken together, OODBL may have therapeutic potential in the treatment of allergic diseases such as asthma.

Keywords: OODBL; asthma; inflammation; leukotriene C4; mast cell degranulation.

MeSH terms

Animals
Anti-Allergic Agents / pharmacology
Anti-Asthmatic Agents / pharmacology
Anti-Inflammatory Agents / pharmacology
Asthma / chemically induced
Asthma / drug therapy
Asthma / metabolism
Bone Marrow Cells / drug effects
Bone Marrow Cells / metabolism
Cell Degranulation / drug effects
Dermatitis, Atopic / chemically induced
Dermatitis, Atopic / drug therapy
Dermatitis, Atopic / metabolism
Disease Models, Animal
Female
Immunoglobulin E / metabolism
Lactones / pharmacology*
Leukotriene C4 / metabolism
Mast Cells / drug effects*
Mast Cells / metabolism
Mice
Mice, Inbred BALB C
Ovalbumin / pharmacology
Phosphorylation / drug effects
Respiratory Hypersensitivity / chemically induced
Respiratory Hypersensitivity / drug therapy*
Respiratory Hypersensitivity / metabolism
Sesquiterpenes / pharmacology*
Signal Transduction / drug effects

Substances

Anti-Allergic Agents
Anti-Asthmatic Agents
Anti-Inflammatory Agents
Lactones
O, O-diacetylbritannilactone
Sesquiterpenes
Leukotriene C4
Immunoglobulin E
Ovalbumin