Evolution of the 3R-MYB Gene Family in Plants

Guanqiao Feng; John Gordon Burleigh; Edward L Braun; Wenbin Mei; William Bradley Barbazuk

doi:10.1093/gbe/evx056

Evolution of the 3R-MYB Gene Family in Plants

Genome Biol Evol. 2017 Apr 1;9(4):1013-1029. doi: 10.1093/gbe/evx056.

Authors

Guanqiao Feng¹, John Gordon Burleigh^{1

2

3}, Edward L Braun^{2

3}, Wenbin Mei², William Bradley Barbazuk^{1

2

3}

Affiliations

¹ Plant Molecular and Cellular Biology Program, University of Florida, Gainesville, FL.
² Department of Biology, University of Florida, Gainesville, FL.
³ Genetics Institute, University of Florida, Gainesville, FL.

Abstract

Plant 3R-MYB transcription factors are an important subgroup of the MYB super family in plants; however, their evolutionary history and functions remain poorly understood. We identified 225 3R-MYB proteins from 65 plant species, including algae and all major lineages of land plants. Two segmental duplication events preceding the common ancestor of angiosperms have given rise to three subgroups of the 3R-MYB proteins. Five conserved introns in the domain region of the 3R-MYB genes were identified, which arose through a step-wise pattern of intron gain during plant evolution. Alternative splicing (AS) analysis of selected species revealed that transcripts from more than 60% of 3R-MYB genes undergo AS. AS could regulate transcriptional activity for some of the plant 3R-MYBs by generating different regulatory motifs. The 3R-MYB genes of all subgroups appear to be enriched for Mitosis-Specific Activator element core sequences within their upstream promoter region, which suggests a functional involvement in cell cycle. Notably, expression of 3R-MYB genes from different species exhibits differential regulation under various abiotic stresses. These data suggest that the plant 3R-MYBs function in both cell cycle regulation and abiotic stress response, which may contribute to the adaptation of plants to a sessile lifestyle.

Keywords: 3R-MYB; abiotic stresses; alternative splicing; cell cycle; gene family evolution; intron evolution.