Treatment strategies for high resveratrol induction in Vitis vinifera L. cell suspension culture

Thu V Vuong; Chris Franco; Wei Zhang

doi:10.1016/j.btre.2014.04.002

Treatment strategies for high resveratrol induction in Vitis vinifera L. cell suspension culture

Biotechnol Rep (Amst). 2014 May 19:1-2:15-21. doi: 10.1016/j.btre.2014.04.002. eCollection 2014 Jun.

Authors

Thu V Vuong^{1

2}, Chris Franco¹, Wei Zhang¹

Affiliations

¹ Department of Medical Biotechnology, School of Medicine, Flinders University, Adelaide 5042, Australia.
² Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, Ontario, Canada.

Abstract

Bioprocesses capable of producing large scales of resveratrol at nutraceutical grade are in demand. This study herein investigated treatment strategies to induce the production of resveratrol in Vitis vinifera L. cell suspension cultures. Among seven investigated elicitors, jasmonic acid (JA), salicylic acid, β-glucan (GLU), and chitosan enhanced the production of intracellular resveratrol manyfold. The combined treatment of JA and GLU increased extracellular resveratrol production by up to tenfold. The application of Amberlite XAD-7 resin for in situ removal and artificial storage of secreted resveratrol further increased resveratrol production by up to four orders of magnitude. The level of resveratrol produced in response to the combined treatment with 200 g/L XAD-7, 10 μM JA and 1 mg/mL GLU was approximately 2400 mg/L, allowing the production of resveratrol at an industrial scale. The high yield of resveratrol is due to the involvement of a number of mechanisms working in concert.

Keywords: Cell suspension culture; Elicitation; In situ adsorption; Resveratrol; Stilbene; Vitis vinifera.