Proposal of a New Estimation Method of Colonization Rate of Arbuscular Mycorrhizal Fungi in the Roots of Chengiopanax sciadophylloides

Seitaro Deguchi; Yosuke Matsuda; Chisato Takenaka; Yuki Sugiura; Hajime Ozawa; Yoshimune Ogata

doi:10.5941/MYCO.2017.45.1.15

Proposal of a New Estimation Method of Colonization Rate of Arbuscular Mycorrhizal Fungi in the Roots of Chengiopanax sciadophylloides

Mycobiology. 2017 Mar;45(1):15-19. doi: 10.5941/MYCO.2017.45.1.15. Epub 2017 Mar 31.

Authors

Seitaro Deguchi¹, Yosuke Matsuda², Chisato Takenaka³, Yuki Sugiura³, Hajime Ozawa⁴, Yoshimune Ogata⁵

Affiliations

¹ Graduate School of Environmental Studies, Nagoya University, Nagoya 464-8601, Japan.
² Graduate School of Bioresources, Mie University, Tsu 514-8507, Japan.
³ Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan.
⁴ Fukushima Prefectural Forestry Research Centre, Koriyama 963-0112, Japan.
⁵ Radioisotope Research Center, Medical Branch, Nagoya University, Nagoya 464-8550, Japan.

Abstract

This study proposed a rapid method to quantify the colonization rate of arbuscular mycorrhizal fungi (AMF) in plant roots. The method involved the use of an image analysis software (WinRHIZO Pro). The colonization rate is defined as the ratio of the fungal body to the plant root area in a micrograph. Three seedlings of Chengiopanax sciadophylloides, a woody species that accumulates radiocesium, were collected from a secondary forest in the Yamakiya district of Kawamata, Fukushima Prefecture during May-September 2014. The colonization of AMF structures was examined under a light microscope, and the percentage of colonization was determined using the WinRHIZO method. The superiority of the new method was verified by comparing with a modified grid-line intersect method. The colonization of AMF was confirmed in all the seedlings, and a significant coefficient of determination (R² = 0.94) was found with both the methods. The results suggested that the WinRHIZO method is reliable for estimating the colonization of AMF in C. sciadophylloides.

Keywords: Image analysis; Microscopy; WinRHIZO; Woody plants.