pH Dependent synthesis of two isomeric dinuclear Cerium(II) complexes: Structures, DNA interactions, cytotoxic activity and apoptotic study

En-Jun Gao; Jun-Qi Su; Hai-Tao Jin; Si-Jia Liu; Fu-Chen Zhao; Yang Meng; Xiao-Yu Ma; Jing Ge; Ya-Guang Sun; Wanzhong Zhang; Ming-Chang Zhu

doi:10.1016/j.jphotobiol.2017.04.010

pH Dependent synthesis of two isomeric dinuclear Cerium(II) complexes: Structures, DNA interactions, cytotoxic activity and apoptotic study

J Photochem Photobiol B. 2017 May:170:173-180. doi: 10.1016/j.jphotobiol.2017.04.010. Epub 2017 Apr 11.

Authors

Affiliations

¹ The Key Laboratory of the Inorganic Molecule-Based Chemistry of Liaoning Province, Department of Coordination Chemistry, Shenyang University of Chemical Technology, Shenyang 110142, China. Electronic address: ejgao@syuct.edu.cn.
² The Key Laboratory of the Inorganic Molecule-Based Chemistry of Liaoning Province, Department of Coordination Chemistry, Shenyang University of Chemical Technology, Shenyang 110142, China.
³ The Key Laboratory of the Inorganic Molecule-Based Chemistry of Liaoning Province, Department of Coordination Chemistry, Shenyang University of Chemical Technology, Shenyang 110142, China. Electronic address: mczhu@syuct.edu.cn.

PMID: 28433873
DOI: 10.1016/j.jphotobiol.2017.04.010

Abstract

Two isomeric dinuclear Cerium(II) complexes 1 and 2, formulated as Ce₂(phen)₂(NO₃)₂(L)₄ [L=phenylacetic acid, phen=1,10-phenanthroline] was synthesized under solvothermal conditions at different pH values. The two complexes were characterized by elemental analysis, IR and single crystal X-ray diffraction. Complexes 1 and 2 were studied the binding with DNA and against cytotoxic activity. Fluorescence analysis indicated that the two complexes can bind to DNA. The changes with different gradient concentration of DNA added into the complexes in absorption spectra show a strongπ-stacking interaction between the complexes and DNA base pairs. The Cerium(II) complexes showed good cytotoxic activity against cancer cell lines, being 2 the most potent complex. Apoptotic studies of the two novel dinuclear complexes showed significant inhibitory rate on cancer cell growth line KB.

Keywords: Apoptotic; Cytotoxic activity; Fluorescent property; UV spectrum.

MeSH terms

Antineoplastic Agents / chemistry*
Antineoplastic Agents / metabolism
Antineoplastic Agents / toxicity
Apoptosis / drug effects
Cell Line, Tumor
Cerium / chemistry*
Coordination Complexes / chemical synthesis*
Coordination Complexes / chemistry
Coordination Complexes / metabolism
Coordination Complexes / toxicity
Crystallography, X-Ray
DNA / chemistry*
DNA / metabolism
HeLa Cells
Humans
Hydrogen Bonding
Hydrogen-Ion Concentration
Isomerism
Molecular Conformation
Phenanthrolines / chemistry
Spectrophotometry, Ultraviolet

Substances

Antineoplastic Agents
Coordination Complexes
Phenanthrolines
Cerium
DNA
1,10-phenanthroline