Detection of Potential Metastatic Prostate Cancer Circulating Biomarkers by Comparison of miRNA Profiles in DU145 Cells and Culture Medium

K A Fomicheva; A I Osip'yants; E N Knyazev; T R Samatov; M Yu Shkurnikov

doi:10.1007/s10517-017-3715-0

Detection of Potential Metastatic Prostate Cancer Circulating Biomarkers by Comparison of miRNA Profiles in DU145 Cells and Culture Medium

Bull Exp Biol Med. 2017 Apr;162(6):792-796. doi: 10.1007/s10517-017-3715-0. Epub 2017 Apr 21.

Authors

K A Fomicheva¹, A I Osip'yants¹, E N Knyazev¹, T R Samatov², M Yu Shkurnikov¹

Affiliations

¹ Р. A. Hertsen Moscow Oncology Research Institute, Branch of the National Medical Research Radiological Centre, The Ministry of Health of the Russian Federation, Moscow, Russia.
² BioClinicum Center, Moscow, Russia.

PMID: 28429232
DOI: 10.1007/s10517-017-3715-0

Abstract

We studied the profile of miRNA secreted into culture medium by DU145 prostate cancer cells and identified a subset of miRNAs characterized by the absence of correlation of their content in the cell and medium, which is likely a result of specific secretion. Three of these miRNA, hsa-miR-4417, hsa-miR-3175, and hsa-miR-6782-5p, exhibit the highest expression and are candidate circulating biomarkers for metastatic activity of prostate cancer. Two of these miRNA are coded by introns of genes linked with genome stability maintenance and chromatin remodeling regulation.

Keywords: DU145; miRNA; prostate cancer.

MeSH terms

Biomarkers, Tumor / genetics*
Cell Line, Tumor
Culture Media / chemistry
Gene Expression Profiling
Gene Expression Regulation, Neoplastic*
Genomic Instability
Humans
Introns
Male
MicroRNAs / genetics*
Neoplasm Invasiveness
Prostate / metabolism*
Prostate / pathology

Substances

Biomarkers, Tumor
Culture Media
MIRN3175 microRNA, human
MicroRNAs