Abstract
Mechanisms of thioredoxin peroxidase secretion by Opisthorchis felineus were studied in vivo and in vitro. Specific antibodies were obtained and used for western blotting and immunohistochemical detection in Syrian hamster model of opisthorchiasis. Secreted thioredoxin peroxidase protein was accumulated in the worm incubation medium under conditions of oxidative stress and in bile duct cells of hamsters with chronic opisthorchiasis.
Keywords:
active oxygen species; cat liver fluke Opisthorchis felineus; excretory-secretory product; opisthorchiasis; thioredoxin peroxidase.
MeSH terms
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Animals
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Antibodies / chemistry
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Antibodies / isolation & purification*
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Bile Ducts / enzymology
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Bile Ducts / parasitology*
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Blotting, Western
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Cloning, Molecular
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Disease Models, Animal
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Escherichia coli / genetics
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Escherichia coli / metabolism
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Fishes / parasitology
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Gene Expression
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Helminth Proteins / agonists
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Helminth Proteins / genetics
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Helminth Proteins / metabolism*
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Hydrogen Peroxide / pharmacology
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Immunohistochemistry
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Mesocricetus / parasitology
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Opisthorchiasis / enzymology
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Opisthorchiasis / parasitology*
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Opisthorchis / drug effects
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Opisthorchis / enzymology*
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Opisthorchis / genetics
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Opisthorchis / isolation & purification
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Oxidative Stress
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Peroxiredoxins / genetics
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Peroxiredoxins / metabolism*
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Rabbits
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Recombinant Proteins / administration & dosage
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Recombinant Proteins / genetics
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Recombinant Proteins / immunology
Substances
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Antibodies
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Helminth Proteins
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Recombinant Proteins
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Hydrogen Peroxide
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Peroxiredoxins